IADR Abstract Archives
Cytotoxicity of Daily Use Toothpastes: In-vitro
Objectives: Toothpastes are daily oral care products, the chemical composition of which is constantly changing due to manufacturer’s competition. The aim of this study was to evaluate the cytotoxic effects of different toothpastes used for prevention and treatment of caries lesions on L929 cells.
Methods: Colgate Total (Colgate-Palmolive, Poland), Sensodyne Pronamel (GlaxoSmithKline, UK), Ipana Total Care (Procter&Gamble, USA), Dant Kanti (Patanjali, India), Gum Gumix (Beka, Turkey) and Tebodont (Dr.Wild&Co.AG,Switzerland) toothpastes were diluted in serum-free medium (50 w/v%) and were shaken vigorously, filter sterilized, and used immediately in the experiments. L929 cells were seeded at density of 104 into each well of a 96-well plate and incubated for 24 hours at 37oC. L929 cells were exposed to dilutions for 24h (n=14). Cell viability was determined by enzime activity (XTT assay). Results were statistically analyzed by one-way ANOVA followed by the Tukey test for post hoc comparisons.
Results: All toothpastes except Gum Gumix have cytotoxic effects on L929 cells (p<0.05). There was no significant difference among Gum Gumix and control groups (p>0.05).
Conclusions: The wide selection of toothpastes and the various ingredients make it difficult for patients to choose the proper toothpaste and complicate the acquisition of dental products by professionals. Toothpastes include active components and these may be harmful effect on oral tissue.
Methods: Colgate Total (Colgate-Palmolive, Poland), Sensodyne Pronamel (GlaxoSmithKline, UK), Ipana Total Care (Procter&Gamble, USA), Dant Kanti (Patanjali, India), Gum Gumix (Beka, Turkey) and Tebodont (Dr.Wild&Co.AG,Switzerland) toothpastes were diluted in serum-free medium (50 w/v%) and were shaken vigorously, filter sterilized, and used immediately in the experiments. L929 cells were seeded at density of 104 into each well of a 96-well plate and incubated for 24 hours at 37oC. L929 cells were exposed to dilutions for 24h (n=14). Cell viability was determined by enzime activity (XTT assay). Results were statistically analyzed by one-way ANOVA followed by the Tukey test for post hoc comparisons.
Results: All toothpastes except Gum Gumix have cytotoxic effects on L929 cells (p<0.05). There was no significant difference among Gum Gumix and control groups (p>0.05).
Conclusions: The wide selection of toothpastes and the various ingredients make it difficult for patients to choose the proper toothpaste and complicate the acquisition of dental products by professionals. Toothpastes include active components and these may be harmful effect on oral tissue.