IADR Abstract Archives
Sublingual administration of HEMA enhances antigen-specific B cell responses to co-administered ovalbumin and Streptococcus mutans
Objectives: The wide use of acrylic based resins in a variety of modern dental procedures calls for a larger knowledge about the material caracteristics and safety, as it is has been reported that leakage of uncured monomers into the oral cavity can cause adverse health effects in dental patients post restorative treatment. The aim of this study was to investigate the ability of 2-hydroxyethyl methacrylate (HEMA) monomers to increase specific systemic immune responses towards bacterial and model antigens, i.e. the adjuvant capacity of HEMA, via the sublingual mucosa.
Methods: Mice (n=8/group) were sublingually administered the common oral bacteria Streptococcus mutans (S. mutans) or Lactobacilli murinus (L. murinus) or the model antigen ovalbumin (OVA) together with HEMA at four weekly occasions. Mice administered S. mutans, L. murinus, OVA or HEMA only served as controls. One week after the last immunization, blood was drawn from the axillary plexus and the experiment was terminated. Serum was prepared and ELISA were performed in order to determine specific antibody responses.
Results: Results show significantly increased anti OVA IgG activity in serum from mice immunized with OVA together with HEMA compared to mice immunized with OVA only. Likewise, S. mutans together with HEMA induced a strong specific IgG production that was significantly higher than the specific response induced by S. mutans only. No significant differences in specific IgG responses were discovered in sera from mice immunized with L. murinus together with HEMA compared to the other groups.
Conclusions: In conclusion, we have shown that HEMA has adjuvant properties when topically co-applied with OVA or S. mutans onto the sublingual mucosa. These results are of clinical relevance as they implicate that leakage of unpolymerized HEMA onto the oral mucosa may enhance immune responses towards common oral bacteria in patients that have undergone dental restoration.
Methods: Mice (n=8/group) were sublingually administered the common oral bacteria Streptococcus mutans (S. mutans) or Lactobacilli murinus (L. murinus) or the model antigen ovalbumin (OVA) together with HEMA at four weekly occasions. Mice administered S. mutans, L. murinus, OVA or HEMA only served as controls. One week after the last immunization, blood was drawn from the axillary plexus and the experiment was terminated. Serum was prepared and ELISA were performed in order to determine specific antibody responses.
Results: Results show significantly increased anti OVA IgG activity in serum from mice immunized with OVA together with HEMA compared to mice immunized with OVA only. Likewise, S. mutans together with HEMA induced a strong specific IgG production that was significantly higher than the specific response induced by S. mutans only. No significant differences in specific IgG responses were discovered in sera from mice immunized with L. murinus together with HEMA compared to the other groups.
Conclusions: In conclusion, we have shown that HEMA has adjuvant properties when topically co-applied with OVA or S. mutans onto the sublingual mucosa. These results are of clinical relevance as they implicate that leakage of unpolymerized HEMA onto the oral mucosa may enhance immune responses towards common oral bacteria in patients that have undergone dental restoration.