IADR Abstract Archives
Immunohistochemical Study of Inflammatory and Regenerative Potential of Dental Pulp
Objectives: Dental pulp in human teeth poses a clinical challenge of healing with the expression of the immunological and angiogenic potential of the tissue. This research aimed to characterize the presence of immune components in the dental pulp and angiogenesis in healthy and carious permanent teeth.
Methods: Method In this study we have examined 30 human teeth under 3 different clinical conditions: healthy teeth, shallow and deep cavities. Teeth were extracted and immediately cut longitudinally; pulp tissue was extirpated and fixed in formalin for 24 hours at 4 °C. The specimens were embedded in paraffin, according to standardized laboratory procedure. Sections were cut at 5 μm thicknesses and stained by the streptavidin-biotin complex immunoperoxidase method. Cells were identified by using the following monoclonal antibodies: HLA-DR, CD45, CD20 and CD34.
Results: The immune response in the unaffected pulp is linked with the presence of antigen-presenting cells, rare T lymphocytes and with single CD34 positive cells. Pulpal immunological response in shallow dentinal caries was characterized by a localized accumulation of HLA-DR antibody-positive cells in the pulp tissue beneath the caries lesion. In the pulp of progressed caries, large number of HLA-DR-positive cells were observed with a marked increase of CD45 and CD20 positive cells. In carious teeth CD34 positive cells subsequently coalesce to form solid vascular cords inside the connective tissue, which later aggregate with the progression of the carious lesion.
Conclusions: These study suggest that immune mechanisms play important role in pulpal infection resistance. Dental caries revealed distinct quantitative and qualitative differences in inflammatory cell infiltration. Presence of CD34 endothelial cells reveals the continuous adjustment of vessels in response to functional needs and play a key role in immune and inflammatory reactions by regulating the dental tissue homeostasis.
Methods: Method In this study we have examined 30 human teeth under 3 different clinical conditions: healthy teeth, shallow and deep cavities. Teeth were extracted and immediately cut longitudinally; pulp tissue was extirpated and fixed in formalin for 24 hours at 4 °C. The specimens were embedded in paraffin, according to standardized laboratory procedure. Sections were cut at 5 μm thicknesses and stained by the streptavidin-biotin complex immunoperoxidase method. Cells were identified by using the following monoclonal antibodies: HLA-DR, CD45, CD20 and CD34.
Results: The immune response in the unaffected pulp is linked with the presence of antigen-presenting cells, rare T lymphocytes and with single CD34 positive cells. Pulpal immunological response in shallow dentinal caries was characterized by a localized accumulation of HLA-DR antibody-positive cells in the pulp tissue beneath the caries lesion. In the pulp of progressed caries, large number of HLA-DR-positive cells were observed with a marked increase of CD45 and CD20 positive cells. In carious teeth CD34 positive cells subsequently coalesce to form solid vascular cords inside the connective tissue, which later aggregate with the progression of the carious lesion.
Conclusions: These study suggest that immune mechanisms play important role in pulpal infection resistance. Dental caries revealed distinct quantitative and qualitative differences in inflammatory cell infiltration. Presence of CD34 endothelial cells reveals the continuous adjustment of vessels in response to functional needs and play a key role in immune and inflammatory reactions by regulating the dental tissue homeostasis.