IADR Abstract Archives
Drug Incorporation of Acrylic Resins - Microbiological and Release Studies
Objectives: The purpose of this study was to evaluate the drug release and antifungal activity against Candida albicans of acrylic reline resins loaded with chlorhexidine.
Methods: Nine experimental group were created according possible combination between acrylic resin (Kooliner, Ufi Gel Hard and Probase Cold) and chlorhexidine incorporation (no drug, chlorhexidine 1% and 2.5% (w/w)) (n=3). For drug release assay, cylinders were stored individually in graduated falcon tubes and covered with artificial saliva at pH=7. The falcons were then placed into an incubator at 37 °C and, at specific time intervals, an aliquot was collected from each falcon, until 28 days. Chlorhexidine content was determined by UV-spectroscopy (255nm). For antifungal activity, resin disks were placed on agar plates inoculated with Candida albicans (ACTT 10231). Positive controls with chlorhexidine and fluconazole were performed. After 48 h at 37 °C, the diameters of inhibition zones by diffusion were measured. Data were analyzed using Kruskal-Wallis and Mann-Whitney tests with Bonferroni correction (α=0.05).
Results: A high initial release was followed by a slower and steadier elution, during the entire study period, in all resins tested. Also, it was demonstrated that the greatest amount of chlorhexidine release occurred within the first 24-48h of incubation. In addition, it was shown that the maximum cumulative release was 1.77%, so only a small amount of initial loaded chlorhexidine was liberated. However, all the materials, even with lower chlorhexidine % (1% w/w), presented a cumulative concentration of chlorhexidine superior than its MIC values for the Candida albicans used in the study. All resins loaded with chlorhexidine showed activity against Candida albicans. Both chlorhexidine release and inhibitory effect on Candida albicans was influenced by drug loading (p<0.05).
Conclusions: Chlorhexidine delivery systems based on acrylic reline resins are a potential approach in the treatment of denture stomatitis.
Methods: Nine experimental group were created according possible combination between acrylic resin (Kooliner, Ufi Gel Hard and Probase Cold) and chlorhexidine incorporation (no drug, chlorhexidine 1% and 2.5% (w/w)) (n=3). For drug release assay, cylinders were stored individually in graduated falcon tubes and covered with artificial saliva at pH=7. The falcons were then placed into an incubator at 37 °C and, at specific time intervals, an aliquot was collected from each falcon, until 28 days. Chlorhexidine content was determined by UV-spectroscopy (255nm). For antifungal activity, resin disks were placed on agar plates inoculated with Candida albicans (ACTT 10231). Positive controls with chlorhexidine and fluconazole were performed. After 48 h at 37 °C, the diameters of inhibition zones by diffusion were measured. Data were analyzed using Kruskal-Wallis and Mann-Whitney tests with Bonferroni correction (α=0.05).
Results: A high initial release was followed by a slower and steadier elution, during the entire study period, in all resins tested. Also, it was demonstrated that the greatest amount of chlorhexidine release occurred within the first 24-48h of incubation. In addition, it was shown that the maximum cumulative release was 1.77%, so only a small amount of initial loaded chlorhexidine was liberated. However, all the materials, even with lower chlorhexidine % (1% w/w), presented a cumulative concentration of chlorhexidine superior than its MIC values for the Candida albicans used in the study. All resins loaded with chlorhexidine showed activity against Candida albicans. Both chlorhexidine release and inhibitory effect on Candida albicans was influenced by drug loading (p<0.05).
Conclusions: Chlorhexidine delivery systems based on acrylic reline resins are a potential approach in the treatment of denture stomatitis.