IADR Abstract Archives
Assessment of microleakage of bioceramic sealer using glucose filtration method
Objectives: The endodontic sealer in combination with thermoplasticized gutta-percha aims to provide a 3D hermetic, fluid-tight seal of the root canal space in order to prevent oral pathogens from colonising and thus reinfecting the root and periapical tissues. The purpose of this in-vitro study was to evaluate the sealing ability of iRoot SP root canal sealer when applied with warm gutta-percha techniques using the fluid filtration method with glucose.
Methods: 52 freshly extracted single-root human teeth were selected for this study. Teeth were divided in 3 experimental groups-iRoot SP+ThermaFil (n=14); iRoot SP+warm vertical condensation (n=14); iRoot SP+injection technique (n=14) and 2 control groups-positive and negative. After the obturation procedures, each tooth was separately positioned in an Eppendorf tube with its apical 7mm protruding through the end. The maxillary end of tube was connected to a syringe containing 0,75ml of 1Mol/L glucose solution. The lower portion of the Eppendorf tube was inserted into another tube containing 0,75ml deionized water so that the apical 3mm was immersed in the water. The maxillary part of the glucose containing syringe was connected a pressure source that created 103kPa for 60 min. After 60 min, a 10 ml aliquot of solution was drawn from each Eppendorf tube and then analysed with glucose kit in a spectrophotometer.
Results: There was no significant difference in glucose leakage in the three experimental groups, although the ThermaFill group demonstrated the lowest levels of glucose concentration, followed by warm vertical condensation and injection technique.
Conclusions: Considering the results in this study iRootSP can provide good sealability when used with warm gutta-percha obturation techniques.
Methods: 52 freshly extracted single-root human teeth were selected for this study. Teeth were divided in 3 experimental groups-iRoot SP+ThermaFil (n=14); iRoot SP+warm vertical condensation (n=14); iRoot SP+injection technique (n=14) and 2 control groups-positive and negative. After the obturation procedures, each tooth was separately positioned in an Eppendorf tube with its apical 7mm protruding through the end. The maxillary end of tube was connected to a syringe containing 0,75ml of 1Mol/L glucose solution. The lower portion of the Eppendorf tube was inserted into another tube containing 0,75ml deionized water so that the apical 3mm was immersed in the water. The maxillary part of the glucose containing syringe was connected a pressure source that created 103kPa for 60 min. After 60 min, a 10 ml aliquot of solution was drawn from each Eppendorf tube and then analysed with glucose kit in a spectrophotometer.
Results: There was no significant difference in glucose leakage in the three experimental groups, although the ThermaFill group demonstrated the lowest levels of glucose concentration, followed by warm vertical condensation and injection technique.
Conclusions: Considering the results in this study iRootSP can provide good sealability when used with warm gutta-percha obturation techniques.