IADR Abstract Archives
Surface Characterization of 3D-Printable Biocompatible Medical Device with Phage Display
Objectives: Currently the application of 3D-printable dental acrylic polymers is limited for the production of accurate drill guides, study cast and orthodontic models. The main composition of 3D-printable acrylate polymers is similar to aesthetic dental filling materials and denture base polymers. It is well known, that the leachable and degradable components of resins often cause allergic reaction at predisposed person. The detailed mechanism of allergy has not clear yet, but it is known that many peptide interactions with peptide motifs can take part, which can be described by directed evolution methods like phage display. Aim of this study is to characterize and to investigate the effect of surface treatment of MED 610 biocompatible 3D-printable polymer on phage display and cell viability.
Methods: In our study, the biocompatible MED 610 (Stratasys, USA) polymer was printed with Objet 30 Orthojet (Stratasys, USA) 3D printer in 3mmx3mmx2 mm prism dimension. One part of the printed specimens were treated according to the manufacturer’s instructions, while the other part of samples were sterilized after the removal of the supporting material. The phage display technique was performed by PhD-7 phage library based on the New England Biolabs protocol. The cell viability tests were done by Alamar Blue assay with two cell lines: a bone osteosarcoma cell line (SAOS-2) and dental pulp stem cells (DPSC).
Results: By phage display analysis, we have found, that TTLLTVS peptide sequence had the highest affinity to the polymer surface. Our results also showed, that cell attaching and proliferation was different on treated and non-treated surface, but the difference is not considerable.
Conclusions: The biocompatible MED 610 polymer was not toxic for the two tested cell lines. However, the number of the attached cells was limited.
This research was supported by TÁMOP 4.2.4. A/2-11-1-2012-0001 ‘National Excellence Program’.
Methods: In our study, the biocompatible MED 610 (Stratasys, USA) polymer was printed with Objet 30 Orthojet (Stratasys, USA) 3D printer in 3mmx3mmx2 mm prism dimension. One part of the printed specimens were treated according to the manufacturer’s instructions, while the other part of samples were sterilized after the removal of the supporting material. The phage display technique was performed by PhD-7 phage library based on the New England Biolabs protocol. The cell viability tests were done by Alamar Blue assay with two cell lines: a bone osteosarcoma cell line (SAOS-2) and dental pulp stem cells (DPSC).
Results: By phage display analysis, we have found, that TTLLTVS peptide sequence had the highest affinity to the polymer surface. Our results also showed, that cell attaching and proliferation was different on treated and non-treated surface, but the difference is not considerable.
Conclusions: The biocompatible MED 610 polymer was not toxic for the two tested cell lines. However, the number of the attached cells was limited.
This research was supported by TÁMOP 4.2.4. A/2-11-1-2012-0001 ‘National Excellence Program’.