IADR Abstract Archives
Biological activity of acid bone lysates: an in vitro study
Objectives: Demineralized bone matrix is considered an allograft produced by exposing native bone to hydrochloric acid. During demineralization, growth factors and other molecules of potential osteogenic activity are presumably released. However, the molecular composition and the cellular response to this acid bone lysate remain unclear. The aim of this study was to investigate the effect of acid bone lysate on cell behavior.
Methods: Porcine cortical bone chips were demineralized and the respective acid lysate harvested. Pellets were obtained after centrifugation of the hydrochloric acid preparation. Following pH neutralization, the remaining bone lysate was filtered sterile. Oral fibroblasts were exposed to the bone lysate. The expression of the TGF-β target genes interleukin 11 (IL11), proteoglycan4 (PRG4), NADPH oxidase 4 (NOX4) were examined by RT-PCR. Signaling was investigated with the TGF-β receptor type I kinase inhibitor SB431542.
Results: We show here that the supernatant obtained after neutralizing the hydrochloric acid was an effective inducers of IL11, PRG4, and NOX4 in oral fibroblasts. Pharmacologic blocking of TGF-β receptor type I kinase with SB431542 greatly reversed the effect on gene expression.
Conclusions: These results demonstrate that acid bone lysates represent a rich source of TGF-β that may be considered in developing regenerative strategies.
Methods: Porcine cortical bone chips were demineralized and the respective acid lysate harvested. Pellets were obtained after centrifugation of the hydrochloric acid preparation. Following pH neutralization, the remaining bone lysate was filtered sterile. Oral fibroblasts were exposed to the bone lysate. The expression of the TGF-β target genes interleukin 11 (IL11), proteoglycan4 (PRG4), NADPH oxidase 4 (NOX4) were examined by RT-PCR. Signaling was investigated with the TGF-β receptor type I kinase inhibitor SB431542.
Results: We show here that the supernatant obtained after neutralizing the hydrochloric acid was an effective inducers of IL11, PRG4, and NOX4 in oral fibroblasts. Pharmacologic blocking of TGF-β receptor type I kinase with SB431542 greatly reversed the effect on gene expression.
Conclusions: These results demonstrate that acid bone lysates represent a rich source of TGF-β that may be considered in developing regenerative strategies.