IADR Abstract Archives
Pulp Capping Materials Modulate the Pulp Inflammatory Activity
Objectives: Due to its location within a rigid and inextensible environment, the pulp regeneration success depends not only on regenerative potential and signals but also on pulp inflammation degree. This work was set to investigate, in vitro, the effect of three pulp capping materials, Biodentine™ (silicate-based), TheraCal® (silicates and resin-based) and Xeno®III (a resin-based bonding system) on modulating the inflammatory response of human pulp fibroblasts.
Methods: Samples of materials were incubated in serum-free culture media to obtain conditioned media (0.05cm2/ml). The effects of these conditioned media were investigated on physically injured pulp fibroblasts stimulated with lipoteichoic acid (LTA) to simulate bacterial infection. The secretion of inflammatory cytokines was quantified by ELISA. Pro-inflammatory activation of vascular endothelium was studied using adhesion of fluorescence (BCECF)-labeled THP-1 cells to endothelial cells (HUVECs) monolayers and THP-1 cells activation was studied using cell adhesion assay.
Results: The anti-inflammatory cytokine TGFβ1 secretion significantly increased with Biodentine™, while it was inhibited by TheraCal® and Xeno®III. The production of IL6 pro-inflammatory cytokine by pulp fibroblasts significantly increased when the cells were incubated with materials’ conditioned media. However, the secretion level with Biodentine™ was lower than that with TheraCal® and Xeno®III. LTA-stimulated and injured fibroblasts induced adhesion of THP-1 to endothelial HUVEC monolayer as well as their activation. When LTA-stimulated and injured fibroblasts were incubated with Biodentine™, THP-1 cell adhesion to HUVEC monolayer as well as the number of activated THP-1 cells drastically decreased. TheraCal® did not affect THP-1 adhesion but decreased THP-1 activation. Finally, Xeno®III significantly increased THP-1 adhesion to HUVEC monolayer.
Conclusions: Taken together, these results performed in vitro show that Biodentine™ has higher anti-inflammatory potential than resin-containing materials such as TheraCal® and Xeno®III.
Methods: Samples of materials were incubated in serum-free culture media to obtain conditioned media (0.05cm2/ml). The effects of these conditioned media were investigated on physically injured pulp fibroblasts stimulated with lipoteichoic acid (LTA) to simulate bacterial infection. The secretion of inflammatory cytokines was quantified by ELISA. Pro-inflammatory activation of vascular endothelium was studied using adhesion of fluorescence (BCECF)-labeled THP-1 cells to endothelial cells (HUVECs) monolayers and THP-1 cells activation was studied using cell adhesion assay.
Results: The anti-inflammatory cytokine TGFβ1 secretion significantly increased with Biodentine™, while it was inhibited by TheraCal® and Xeno®III. The production of IL6 pro-inflammatory cytokine by pulp fibroblasts significantly increased when the cells were incubated with materials’ conditioned media. However, the secretion level with Biodentine™ was lower than that with TheraCal® and Xeno®III. LTA-stimulated and injured fibroblasts induced adhesion of THP-1 to endothelial HUVEC monolayer as well as their activation. When LTA-stimulated and injured fibroblasts were incubated with Biodentine™, THP-1 cell adhesion to HUVEC monolayer as well as the number of activated THP-1 cells drastically decreased. TheraCal® did not affect THP-1 adhesion but decreased THP-1 activation. Finally, Xeno®III significantly increased THP-1 adhesion to HUVEC monolayer.
Conclusions: Taken together, these results performed in vitro show that Biodentine™ has higher anti-inflammatory potential than resin-containing materials such as TheraCal® and Xeno®III.