IADR Abstract Archives
Pulp Healing Under Pulp Capping Materials in vitro
Objectives: Deep pulp lesions often lead to odontoblast and pulp fibroblast destruction. The initiation of pulp/dentin regeneration requires the presence of dental pulp stem cells and their migration from the perivascular niches to the injury site. This also requires proliferation of pulp fibroblasts at the injured site or their recruitment from adjacent sites to replace the missing tissue.
This work was designed to investigate, in vitro, the effect of Biodentine™ (silicate-based), TheraCal® (silicates and resin-based) and Xeno®III (a resin-based bonding system) on the pulp healing potential.
Methods: Samples of materials were incubated in serum free culture medium to obtain conditioned media (0.05cm2/ml). The effect of these conditioned media was investigated on injured pulp fibroblasts incubated with lipoteichoic acid (LTA) to simulate bacterial infection. The secretion of the Fibroblast Growth factor FGF2 was quantified by ELISA. Proliferation of pulp fibroblasts was quantified using the MTT assay and healing was studied in vitro using the scratch wound healing assay.
Results: FGF2 secretion significantly increased with the three materials’ conditioned media. However, Biodentine™ induced a significantly higher secretion of this growth factor. When pulp fibroblasts were grown in supernatants of LTA-stimulated and injured pulp fibroblasts in Biodentine™ conditioned media, an increased proliferation was observed. By contrast, this proliferation decreased with TheraCal® and Xeno®III conditioned media. Moreover, supernatants of LTA-stimulated and injured fibroblasts had no effect on the healing process with TheraCal® or Xeno®III conditioned media, while Biodentine™ induced the healing process using the scratch healing assay.
Conclusions: Taken together, these results show that Biodentine™ has a higher pulp healing potential than TheraCal® or Xeno®III.
This work was designed to investigate, in vitro, the effect of Biodentine™ (silicate-based), TheraCal® (silicates and resin-based) and Xeno®III (a resin-based bonding system) on the pulp healing potential.
Methods: Samples of materials were incubated in serum free culture medium to obtain conditioned media (0.05cm2/ml). The effect of these conditioned media was investigated on injured pulp fibroblasts incubated with lipoteichoic acid (LTA) to simulate bacterial infection. The secretion of the Fibroblast Growth factor FGF2 was quantified by ELISA. Proliferation of pulp fibroblasts was quantified using the MTT assay and healing was studied in vitro using the scratch wound healing assay.
Results: FGF2 secretion significantly increased with the three materials’ conditioned media. However, Biodentine™ induced a significantly higher secretion of this growth factor. When pulp fibroblasts were grown in supernatants of LTA-stimulated and injured pulp fibroblasts in Biodentine™ conditioned media, an increased proliferation was observed. By contrast, this proliferation decreased with TheraCal® and Xeno®III conditioned media. Moreover, supernatants of LTA-stimulated and injured fibroblasts had no effect on the healing process with TheraCal® or Xeno®III conditioned media, while Biodentine™ induced the healing process using the scratch healing assay.
Conclusions: Taken together, these results show that Biodentine™ has a higher pulp healing potential than TheraCal® or Xeno®III.