IADR Abstract Archives
Pathogenesis of Porphyromonas gulae in humans
Objectives: We investigated the distribution of P. gulae in dogs and their owners using oral specimens subjected to a molecular biological method to investigate the possibility of dog-owner transmission of P. gulae.
Methods: Porphyromonas gulae, a novel oral bacterial species, is often isolated from the gingival sulcus of various animals including dogs and cats. Dental plaque specimens from the dogs and their owners were collected from the gingival margin of the buccal side of the maxillary molar region using sterile cotton swabs. A 16S rRNA-based PCR detection method was used to determine the distribution of P. gulae. The adherence to human gingival fibroblast and cytokine production were examined. Special pathogen-free 3-week-old male Sprague-Dawley rats were orally infected with P. gulae ATCC 51700 and alveolar bone loss was measured. The study has been conducted under the approval from the Ethical committee for Clinical Research of Kanagawa Dental University (No. 335).
Results: P. gulae was detected from dental plaque specimens of three owners by P. gulae species specific primes. P. gulae ATCC 51700 adhered to human cultured cells and induced IL-1 and TNF- production in BALB/c mouse peritoneal macrophages. Rats orally infected with P. gulae exhibited significant alveolar bone loss as compared with that of the sham-infected rats.
Conclusions: Its related strains of human origin, Porphyromonas gingivalis, is known to have fimbriae that play an important role in adherence of the organisms to oral epithelial cells as the initial step in the progression of periodontitis. P. gulae could be transmitted humans from their dogs. P. gulae-positive dog may act as a source of infection for human. These findings suggest that binding sites involved in the interaction with the human host cell present in P. gulae. Moreover, P. gulae play important role in the pathogenesis of periodontal disease.
Methods: Porphyromonas gulae, a novel oral bacterial species, is often isolated from the gingival sulcus of various animals including dogs and cats. Dental plaque specimens from the dogs and their owners were collected from the gingival margin of the buccal side of the maxillary molar region using sterile cotton swabs. A 16S rRNA-based PCR detection method was used to determine the distribution of P. gulae. The adherence to human gingival fibroblast and cytokine production were examined. Special pathogen-free 3-week-old male Sprague-Dawley rats were orally infected with P. gulae ATCC 51700 and alveolar bone loss was measured. The study has been conducted under the approval from the Ethical committee for Clinical Research of Kanagawa Dental University (No. 335).
Results: P. gulae was detected from dental plaque specimens of three owners by P. gulae species specific primes. P. gulae ATCC 51700 adhered to human cultured cells and induced IL-1 and TNF- production in BALB/c mouse peritoneal macrophages. Rats orally infected with P. gulae exhibited significant alveolar bone loss as compared with that of the sham-infected rats.
Conclusions: Its related strains of human origin, Porphyromonas gingivalis, is known to have fimbriae that play an important role in adherence of the organisms to oral epithelial cells as the initial step in the progression of periodontitis. P. gulae could be transmitted humans from their dogs. P. gulae-positive dog may act as a source of infection for human. These findings suggest that binding sites involved in the interaction with the human host cell present in P. gulae. Moreover, P. gulae play important role in the pathogenesis of periodontal disease.