IADR Abstract Archives
Human dental pulp cell response to experimental tricalcium silicate cement
Objectives: To investigate the effect of the experimental tricalcium silicate cement ‘TCS 50’ on proliferation and odontogenic differentiation of human dental pulp cells (hDPCs).
Methods: Cylindrical cement disks (diameter: 22.1 mm; thickness: 1 mm) were prepared; each disk was immediately exposed to 3-ml α-MEM culture medium. Cement extracts were daily collected and three dilutions (1:2, 1:4, 1:10) were prepared. Proliferation of hDPCs cultured with undiluted/diluted cement extracts was evaluated at day 1, 4 and 7 using an XTT assay. The odontogenic differentiation of hDPCs was evaluated using real-time reverse-transcriptase polymerase chain reaction. hDPCs were exposed to 1:4 diluted cement extract and gene expressions of the cell odontogenic differentiation markers alkaline phosphatase (ALP), dentin sialophosphoprotein (DSPP) and osteocalcin (OC) were assessed at day 4, 10 and 14. The commercial cements ProRoot MTA (Denstply) and TheraCal LC (Bisco), and α-MEM culture medium served as controls.
Results: The proliferation trend of cells exposed to undiluted and diluted TCS 50 extracts resembled closely to that of the medium control (p>0.05). ProRoot MTA also showed no adverse effect on cell proliferation, while a clear decline was detected for TheraCal LC extracts (p<0.05). Regarding cell odontogenic differentiation, all gene expressions for TCS 50 extract were significantly lower than for the control at day 4 (p<0.05). However, at day 10, all gene expressions were upregulated to a higher level than for the control (p<0.05). At day 14, ALP expression was downregulated, while DSPP and OC expressions were still higher than the control (p<0.05). For ProRoot MTA and TheraCal LC, all gene expressions were lower than the control at all time intervals (p<0.05); the only exception was the 14-day OC expression for ProRoot MTA, being similar as the control.
Conclusions: TCS 50 revealed no effect on hDPC proliferation, while it promoted cell odontogenic differentiation.
Methods: Cylindrical cement disks (diameter: 22.1 mm; thickness: 1 mm) were prepared; each disk was immediately exposed to 3-ml α-MEM culture medium. Cement extracts were daily collected and three dilutions (1:2, 1:4, 1:10) were prepared. Proliferation of hDPCs cultured with undiluted/diluted cement extracts was evaluated at day 1, 4 and 7 using an XTT assay. The odontogenic differentiation of hDPCs was evaluated using real-time reverse-transcriptase polymerase chain reaction. hDPCs were exposed to 1:4 diluted cement extract and gene expressions of the cell odontogenic differentiation markers alkaline phosphatase (ALP), dentin sialophosphoprotein (DSPP) and osteocalcin (OC) were assessed at day 4, 10 and 14. The commercial cements ProRoot MTA (Denstply) and TheraCal LC (Bisco), and α-MEM culture medium served as controls.
Results: The proliferation trend of cells exposed to undiluted and diluted TCS 50 extracts resembled closely to that of the medium control (p>0.05). ProRoot MTA also showed no adverse effect on cell proliferation, while a clear decline was detected for TheraCal LC extracts (p<0.05). Regarding cell odontogenic differentiation, all gene expressions for TCS 50 extract were significantly lower than for the control at day 4 (p<0.05). However, at day 10, all gene expressions were upregulated to a higher level than for the control (p<0.05). At day 14, ALP expression was downregulated, while DSPP and OC expressions were still higher than the control (p<0.05). For ProRoot MTA and TheraCal LC, all gene expressions were lower than the control at all time intervals (p<0.05); the only exception was the 14-day OC expression for ProRoot MTA, being similar as the control.
Conclusions: TCS 50 revealed no effect on hDPC proliferation, while it promoted cell odontogenic differentiation.