IADR Abstract Archives
Cytotoxicity Comparison of 2 Bulk Fill Resin-Composites
Objectives: The aim of this study is to compare the cytotoxicity in vitro of two different Bulk fill composite-resins: Filtek™ Bulk Fill (3M™ ESPE) and Tetric EvoCeram® Bulk Fill (Ivoclar Vivadent®).
Methods: Discs of each resin (4mmx4mm) (2 groups, n=10/grp) were photopolymerized and incubated in Dulbecco's modified Eagle Medium nutrient mixture (DMEM) (24h, 37°C) to yield extracts with eluted components. 3T3 fibroblasts were exposed to different dilutions of the extracts (10%, 50%, 100%) (24h, 37°C). Cytotoxicity positive control (70% ethanol/DMEM) and negative control (cells in DMEM) were used. Cell viability was quantified by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. Cell viability data were statistically analysed by using a two-way ANOVA, at a significance level of 5%, considering resin and extract concentration as main effects.
Results: All dilutions of added resin extracts caused cell death (>80%). No statistically significant differences were observed between the cytotoxicity of the tested resins (p=0.154). In both resins, there was a decrease in cell viability when increasing extract concentration (p<0.001). No interaction effect was identified between resin and extract concentration (p=0.809).
Conclusions: In the first 24h, there was release of cytotoxic components in both resins. They were cytotoxic in all dilutions tested, although no significant differences were observed between them. The identification of toxic components is important to avoid their use in dentistry.
Methods: Discs of each resin (4mmx4mm) (2 groups, n=10/grp) were photopolymerized and incubated in Dulbecco's modified Eagle Medium nutrient mixture (DMEM) (24h, 37°C) to yield extracts with eluted components. 3T3 fibroblasts were exposed to different dilutions of the extracts (10%, 50%, 100%) (24h, 37°C). Cytotoxicity positive control (70% ethanol/DMEM) and negative control (cells in DMEM) were used. Cell viability was quantified by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. Cell viability data were statistically analysed by using a two-way ANOVA, at a significance level of 5%, considering resin and extract concentration as main effects.
Results: All dilutions of added resin extracts caused cell death (>80%). No statistically significant differences were observed between the cytotoxicity of the tested resins (p=0.154). In both resins, there was a decrease in cell viability when increasing extract concentration (p<0.001). No interaction effect was identified between resin and extract concentration (p=0.809).
Conclusions: In the first 24h, there was release of cytotoxic components in both resins. They were cytotoxic in all dilutions tested, although no significant differences were observed between them. The identification of toxic components is important to avoid their use in dentistry.