IADR Abstract Archives
Implant Surfaces manufactured by multi-material laser sintering – In vitro study
Objectives: The aim of this study was to evaluate the behavior of human fetal osteoblasts (hFOB1.19) in contact with Polyetheretherketone (PEEK) or Zirconia-based dental implant biomaterials produced using a new manufacturing procedure comparing to Titanium as a gold standard material.
Methods: PEEK and Zirconia discs were produced by a novel pressing-sintering technique. Commercially pure grade IV Titanium (Ti) discs were used as control and homogeneous surface roughness was ensured for all samples (n=8 for each group). Human osteoblasts were cultured on discs for 15 days using previously established methods. Morphology and cellular adhesion was observed using scanning electron microscopy (SEM) after 24h incubation. Cell viability and proliferation were evaluated at pre-defined time-points (1,3,7 and 14 days) using a commercial resazurin-based method. Alkaline phosphatase (ALP) activity was evaluated at 7 and 14 days. All results were presented as mean fluorescence intensity (AU) ± standard deviation (SD). Mineralization patterns were evaluated using hydroxyapatite fluorescence staining. Group comparisons were tested using Anova (Tukey’s post-hoc) using appropriate statistical software and significance was set p<0.05.
Results: The results showed cell adhesion on all test materials and visible intercellular extensions on Zirconia group for 24h growth. Cell viability increased over time demonstrating cell proliferation, which was higher on PEEK (86.4±11.3 AU) and Zirconia (90.8±29.9AU) groups (p<0.05) when comparing to Titanium (41.5±2.9AU) at 14 days.
All groups presented increased ALP activity values over time, however, group comparisons at each time-point failed to be significant.
Qualitative analysis of mineralization patterns with fluorescence demonstrated an increase in mineral content over time, which was more apparent in the Zirconia group. Mineralization results for PEEK group were excluded due to intrinsic material fluorescence.
Conclusions: The new production technique for Zirconia and PEEK-based materials showed increased osteoblast viability, proliferation and matrix mineralization in vitro, when compared to Titanium.
Methods: PEEK and Zirconia discs were produced by a novel pressing-sintering technique. Commercially pure grade IV Titanium (Ti) discs were used as control and homogeneous surface roughness was ensured for all samples (n=8 for each group). Human osteoblasts were cultured on discs for 15 days using previously established methods. Morphology and cellular adhesion was observed using scanning electron microscopy (SEM) after 24h incubation. Cell viability and proliferation were evaluated at pre-defined time-points (1,3,7 and 14 days) using a commercial resazurin-based method. Alkaline phosphatase (ALP) activity was evaluated at 7 and 14 days. All results were presented as mean fluorescence intensity (AU) ± standard deviation (SD). Mineralization patterns were evaluated using hydroxyapatite fluorescence staining. Group comparisons were tested using Anova (Tukey’s post-hoc) using appropriate statistical software and significance was set p<0.05.
Results: The results showed cell adhesion on all test materials and visible intercellular extensions on Zirconia group for 24h growth. Cell viability increased over time demonstrating cell proliferation, which was higher on PEEK (86.4±11.3 AU) and Zirconia (90.8±29.9AU) groups (p<0.05) when comparing to Titanium (41.5±2.9AU) at 14 days.
All groups presented increased ALP activity values over time, however, group comparisons at each time-point failed to be significant.
Qualitative analysis of mineralization patterns with fluorescence demonstrated an increase in mineral content over time, which was more apparent in the Zirconia group. Mineralization results for PEEK group were excluded due to intrinsic material fluorescence.
Conclusions: The new production technique for Zirconia and PEEK-based materials showed increased osteoblast viability, proliferation and matrix mineralization in vitro, when compared to Titanium.