IADR Abstract Archives
A-PRF Revascularization of Immature Teeth: Success Rate after 6 Months
Objectives: A-PRF (Advanced Platelet-Rich Fibrin) is widely used biomaterial in oral maxillofacial surgical procedures. Many studies dealing with revascularization of young permanent teeth using Ca(OH)2 showed very unpredictable results. Aim of this study was to analyze success rate of revascularization after 6 months using A-PRF autologous biomaterial.
Methods: Material comprised 10 immature one-rooted teeth with pulp necrosis. 10ml of venous blood was collected from each patient, centrifugated for A-PRF protocol(1300rpm/8min,PRF DUO Process For PRF, France). Teeth were prepared according to classic revascularization protocol (Ca(OH)2 and tri-antibiotic paste). A-PRF biomaterial was placed in root canal and cavity was sealed with GlassCarbomer(GCP Dental) material. Analysis of tooth vitality was performed using cold test, electrical test, Laser-Doppler flowmetry and radiographic analysis after 1, 3 and 6 months.
Results: Cold test was positive in 50% cases after 1 and 3 months and 70% after 6mths. 60% patients reacted on electrical test after 1mth, 50% after 3mth and 60% after 6mths. Laser-Doppler flowmetry showed Flux in 100% cases. Radiographic analysis: At the beginning of treatment periapical lesion was present in 40% patients. In 3 cases lesion resolved after 3mths and in 4th case after 6mths. In one case of avulsed tooth periapical lesion occurred between 3rd and 6th month. Radiographic root elongation was measured in 20%(Χ=0,35mm) after 1mth, in 40%(Χ=0,9mm) after 3mths and in 50%(Χ=1,2mm) after 6mths. Dental wall thickening was measured in 10%(Χ=0,2mm) after 1mnth, 30%(Χ=0,3mm) after 3mths and 30%(Χ=0,53mm) after 6mths. Apical closure was noted in 40%(Χ=0,225mm) after 1mth, 50%(Χ=0,46mm) after 3mths and 60%(Χ=0,517mm) after 6mths.
Conclusions: It can be concluded that proposed method of revascularization showed high percentage of success and could be used in clinical practice. Further research is needed to get more reliable results.
Methods: Material comprised 10 immature one-rooted teeth with pulp necrosis. 10ml of venous blood was collected from each patient, centrifugated for A-PRF protocol(1300rpm/8min,PRF DUO Process For PRF, France). Teeth were prepared according to classic revascularization protocol (Ca(OH)2 and tri-antibiotic paste). A-PRF biomaterial was placed in root canal and cavity was sealed with GlassCarbomer(GCP Dental) material. Analysis of tooth vitality was performed using cold test, electrical test, Laser-Doppler flowmetry and radiographic analysis after 1, 3 and 6 months.
Results: Cold test was positive in 50% cases after 1 and 3 months and 70% after 6mths. 60% patients reacted on electrical test after 1mth, 50% after 3mth and 60% after 6mths. Laser-Doppler flowmetry showed Flux in 100% cases. Radiographic analysis: At the beginning of treatment periapical lesion was present in 40% patients. In 3 cases lesion resolved after 3mths and in 4th case after 6mths. In one case of avulsed tooth periapical lesion occurred between 3rd and 6th month. Radiographic root elongation was measured in 20%(Χ=0,35mm) after 1mth, in 40%(Χ=0,9mm) after 3mths and in 50%(Χ=1,2mm) after 6mths. Dental wall thickening was measured in 10%(Χ=0,2mm) after 1mnth, 30%(Χ=0,3mm) after 3mths and 30%(Χ=0,53mm) after 6mths. Apical closure was noted in 40%(Χ=0,225mm) after 1mth, 50%(Χ=0,46mm) after 3mths and 60%(Χ=0,517mm) after 6mths.
Conclusions: It can be concluded that proposed method of revascularization showed high percentage of success and could be used in clinical practice. Further research is needed to get more reliable results.