IADR Abstract Archives
In vitro and in vivo assessment of ξ-Polycaprolactone membrane functionalized with ibuprofen on periodontal wound healing
Objectives: Inflammation worsens periodontal wound healing. The control of inflammation promotes wound closure and tissue regeneration. In this study, the anti-inflammatory properties of an ibuprofen-functionalized ξ-polycaprolactone membrane (IBU-PCL) on gingival epithelial cells and fibroblasts were investigated in vitro and periodontal healing was assessed in vivo.
Methods: IBU-PCL membrane was synthesized by electrospinning using core-shell technique. Membrane characterization and release of ibuprofen from the scaffold were investigated with Scanning Electron Microscopy and Transmission Electron Microscopy respectively. Evaluation of anti-inflammatory properties was performed on Pg-LPS stimulated oral epithelial cells (TERT-2) and fibroblasts through RT-qPCR. Impact on cells’ metabolic activity and proliferation was measured using AlamarBlue and immunofluorescent assays. IBU-PCL membrane was also evaluated in vivo in a periodontitis-induced mouse model after 22 days through histomorphometric analysis.
Results: In vitro, the proliferation rate of gingival cells was reduced significantly by the IBU-PCL membrane. Moreover, it decreased significantly the relative gene expression of inflammatory mediators (COX-2, IL-8 (4-fold)) at 24 hours in both cell types. Furthermore, the gene expression of extra cellular matrix (Col-IV, Fibronectin-1, Integrin α3β1, Laminin-5) was reduced with a drastic reduction of 12 and 6 folds for laminin at 6 and 12 hours respectively in epithelial cells. The impact was, generally, stronger on epithelial cells compared to fibroblasts. In vivo, histomorphometric analysis showed that treatment with IBU-PCL membrane increased connective tissue attachment on root surface.
Conclusions: This study showed that IBU-PCL membrane decreased the proliferative and inflammatory response of stimulated human gingival cells. IBU-PCL maybe an efficient local drug delivery scaffold that could allow time-controlled diffusion of anti-inflammatory drugs during wound healing with potential pro-regenerative effect.
Methods: IBU-PCL membrane was synthesized by electrospinning using core-shell technique. Membrane characterization and release of ibuprofen from the scaffold were investigated with Scanning Electron Microscopy and Transmission Electron Microscopy respectively. Evaluation of anti-inflammatory properties was performed on Pg-LPS stimulated oral epithelial cells (TERT-2) and fibroblasts through RT-qPCR. Impact on cells’ metabolic activity and proliferation was measured using AlamarBlue and immunofluorescent assays. IBU-PCL membrane was also evaluated in vivo in a periodontitis-induced mouse model after 22 days through histomorphometric analysis.
Results: In vitro, the proliferation rate of gingival cells was reduced significantly by the IBU-PCL membrane. Moreover, it decreased significantly the relative gene expression of inflammatory mediators (COX-2, IL-8 (4-fold)) at 24 hours in both cell types. Furthermore, the gene expression of extra cellular matrix (Col-IV, Fibronectin-1, Integrin α3β1, Laminin-5) was reduced with a drastic reduction of 12 and 6 folds for laminin at 6 and 12 hours respectively in epithelial cells. The impact was, generally, stronger on epithelial cells compared to fibroblasts. In vivo, histomorphometric analysis showed that treatment with IBU-PCL membrane increased connective tissue attachment on root surface.
Conclusions: This study showed that IBU-PCL membrane decreased the proliferative and inflammatory response of stimulated human gingival cells. IBU-PCL maybe an efficient local drug delivery scaffold that could allow time-controlled diffusion of anti-inflammatory drugs during wound healing with potential pro-regenerative effect.