Efficacy of Doxycycline Release Collagen Membrane on Contaminated Defects in Rat Tibiae

Objectives: The aim of this study was to investigate the osteogenesis potential and the antibacterial effect of a doxycycline releasing collagen membrane in surgically created and contaminated defects in rat tibiae.
Methods: Defects were created in 30 rats that were randomly divided into five groups: Control group 1; Control group 2 (defect contaminated by Porphyromonas gingivalis ; Xenogreft group (defect contaminated by P. Gingivalis and filled with bone graft); Pancol group (defect contaminated by P. Gingivalis, filled with bone graft and covered by Pancol); Doxicoll group (defect contaminated by P. Gingivalis filled with bone graft and covered by Doxicoll). Animals were sacrificed post surgically on the 14^th day for microbiologic evaluation and on the 28^th day for histopathological evaluation. The Kruskal Wallis test was used forcomparing different parameters between the groups.The Mann Whitney U test was used for post hoc determinations. The chi-square test was used to compare qualitative data. The significance level was set at p<0.05.
Results: The degree of osteogenesis in the Doxicoll group (1.80±0.45) was found to be significantly higher than that of the Pancoll group (0.33±0.52) (p:0.007; p<0.01). Furthermore, the lowest bacterial count (3x10⁴ CFU) was observed in the Doxicoll group compared to all other groups.
Conclusions: Within the limitations of this study, Doxicoll could be a favourable material for the enhancement of bone formation and limiting P. Gingivalis infections following GBR procedures in a rat model.