IADR Abstract Archives
Biomarkers in gingival crevicular fluid for diagnosis of Asymptomatic Apical Periodontitis
Objectives: To assess levels and diagnostic accuracy of a set of potential biomarkers in Gingival Crevicular Fluid (GCF) of subjects with Asymptomatic Apical Periodontitis (AAP).
Methods: In this cross-sectional study, the GCF samples were obtained from tooth sites of subjects with diagnosis of AAP (n=44) and healthy controls (n=31). Total Protein Concentration (TPC) was determined by the Bicinchoninate method. The gelatinolytic activity of Matrix Metalloproteinases (MMPs) -2 and -9 was determined by zymography and densitometric analysis. The MMP-8 levels were determined by ELISA and IFMA. The direct bone markers (DKK-1, Osteonectin, Periostin, TRAP-5 and OPG) were determined by the multiplex quantitative method. For comparisons among groups, the Mann-Whitney tests was used. For evaluating markers of interest such as diagnostic accuracy tests, ROC curves were made and the area under the curve (AUC) was calculated. Statistical analysis was performed using linear mixed-effects models and Stata V12 software and the statistical significance was considered with a value of p<0.05.
Results: The proenzyme, active form, and activation rate of MMP-9 and levels of MMP-8 were significantly higher in tooth sites of subjects with AAP when compared to healthy subjects (p<0.05). The markers' diagnostic accuracy analysis revealed that tooth sites with AAP, the MMP-8 and active MMP-9 marker showed a high diagnostic accuracy (AUC>0.9).
Conclusions: GCF composition is modified by AAP. MMP-8 and active MMP-9 have diagnostic value for AAP.
Methods: In this cross-sectional study, the GCF samples were obtained from tooth sites of subjects with diagnosis of AAP (n=44) and healthy controls (n=31). Total Protein Concentration (TPC) was determined by the Bicinchoninate method. The gelatinolytic activity of Matrix Metalloproteinases (MMPs) -2 and -9 was determined by zymography and densitometric analysis. The MMP-8 levels were determined by ELISA and IFMA. The direct bone markers (DKK-1, Osteonectin, Periostin, TRAP-5 and OPG) were determined by the multiplex quantitative method. For comparisons among groups, the Mann-Whitney tests was used. For evaluating markers of interest such as diagnostic accuracy tests, ROC curves were made and the area under the curve (AUC) was calculated. Statistical analysis was performed using linear mixed-effects models and Stata V12 software and the statistical significance was considered with a value of p<0.05.
Results: The proenzyme, active form, and activation rate of MMP-9 and levels of MMP-8 were significantly higher in tooth sites of subjects with AAP when compared to healthy subjects (p<0.05). The markers' diagnostic accuracy analysis revealed that tooth sites with AAP, the MMP-8 and active MMP-9 marker showed a high diagnostic accuracy (AUC>0.9).
Conclusions: GCF composition is modified by AAP. MMP-8 and active MMP-9 have diagnostic value for AAP.