Evaluaton of In Vitro Genotoxicity of MTA Fillapex

Objectives: The aim of the present study was to evaluate the genotoxicity and cytotoxicity of MTA Fillapex and AH Plus root canal sealers with Cytokinesis-block micronucleus cytome assay
Methods: Ten mL of blood was taken from healthy volunteers and lymphocytes cultures were obtained from those samples. Different concentrations of MTA Fillapex and AH Plus root canal sealers were added to cultures and after then the cells with micronucleus (MN), with binucleus, the number of metaphases, nucleoplasmic bridge (NBP) and nucleoplasmic buds (NBud) were counted. A thousand cells were counted through the preparats and apopthic, necrotic cells were counted.
Results: MTA Fillapex root canal sealer's toxicity was raised by the increase of dosage. But except NBP parameter MTA Fillapex showed significantly less toxicity then positive control group. For NBP parameter there was no significant difference between MTA Fillapex and positive control. AH Plus sealer showed toxicity during the polymerization period.
Conclusions: MTA Fillapex paste showed less cytotoxicity than AH Plus paste.