IADR Abstract Archives
Effect of Implantation of Dental Materials on the Serum Oxidant and Antioxidant Status in Rats
Objectives: Objective: To measure and evaluate the total oxidative stress and total antioxidant capacity of the AhPlus, Obtuseal, Micro Mega Mineral Trioxide Aggregate (MM-MTA), Biodentine (BD) materials in serum samples after the implantation into the subcutanous tissue of rats.
Methods: Materials and Methods: AhPlus, Obtuseal, MM-MTA, BA and BD materials were implanted into the subcutaneous tissue of 26 Wistar albino rats and 6 more animals used as a control group which had no operation. At the end of the 45 days animals were sacrified by cervical dislocation after anesthesia and blood samples were obtained. The serum samples were separated from the cells by centrifugation at 3000 rpm for 15 min and stored at -20 °C and used for the analysis of total oxidant status (TOS) and total antioxidant status (TAS). The percentage ratio of TOS:TAS was used to calculate the OSI, a potential indicator of the degree of oxidative stress. Kruskal-Wallis and Connover test analyses were performed with IBM SPSS statistics 22.0 program and p<0.05 was considered statistically significant.
Results: Results: There were no significant difference between the AhPlus, Obtuseal, MM-MTA, BD groups and control group. But for the TOS and OSI values there were statistically significant difference between MM-MTA and BD groups, MM-MTA and Obtuseal groups, BD and AhPlus groups and also Obtuseal and AhPlus groups.
Conclusions: Conclusion: The presence of dental materials in the subcutaneous tissue seems not to affect systemic TOS, TAS and OSI values in rats.
Methods: Materials and Methods: AhPlus, Obtuseal, MM-MTA, BA and BD materials were implanted into the subcutaneous tissue of 26 Wistar albino rats and 6 more animals used as a control group which had no operation. At the end of the 45 days animals were sacrified by cervical dislocation after anesthesia and blood samples were obtained. The serum samples were separated from the cells by centrifugation at 3000 rpm for 15 min and stored at -20 °C and used for the analysis of total oxidant status (TOS) and total antioxidant status (TAS). The percentage ratio of TOS:TAS was used to calculate the OSI, a potential indicator of the degree of oxidative stress. Kruskal-Wallis and Connover test analyses were performed with IBM SPSS statistics 22.0 program and p<0.05 was considered statistically significant.
Results: Results: There were no significant difference between the AhPlus, Obtuseal, MM-MTA, BD groups and control group. But for the TOS and OSI values there were statistically significant difference between MM-MTA and BD groups, MM-MTA and Obtuseal groups, BD and AhPlus groups and also Obtuseal and AhPlus groups.
Conclusions: Conclusion: The presence of dental materials in the subcutaneous tissue seems not to affect systemic TOS, TAS and OSI values in rats.