IADR Abstract Archives
Are titanium fixation screws biocompatible? An in vitro study
Objectives: To evaluate the cytotoxic effects of titanium fixation screws on human gingival fibroblasts (HGF) and mouse osteoblasts (MC3T3-E1).
Methods: The fixation screws selected were LEIBENGER, TRIMED, and LORENZ. Five samples were used for each group. Fixation System were each incubated in DMEM for 72 hours according to ISO 10993-5 standards. A real-time cell analyzer was used to evaluate cell survival. After seeding 200 µL cell suspensions into the wells of an E-plate 96, HGF and MC3T3-E1 cells were treated with the bioactive components released by the materials and monitored every 15 minutes for 96 hours. Statistical significance was determined using one-way analysis of variance and Tukey-Kramer tests.
Results: After 48 hours of elution, there were significant differences in the HGF cell-index values between the untreated control group and the study groups (P < 0.001) . In addition, the HGF cell-index values of the Leibinger (P < 0.001), Trimed (p < 0.01), and Lorenz (P < 0.01) were significantly lower than that of the untreated control after 72 hours.
When evaluated the after 96 hours, while there were is significant differences among the HGF cell index values of the control and Leibinger (p < 0.01) and Trimed (p < 0.05) groups . There is no differences between the of the control and Lorenz (p>0.05) groups.
Conclusions: Titanium based fixation screws caused a significant decrease in MC3T3-E1 cell viability, there was no adverse effect in all tested materials on human gingival fibroblasts.
Methods: The fixation screws selected were LEIBENGER, TRIMED, and LORENZ. Five samples were used for each group. Fixation System were each incubated in DMEM for 72 hours according to ISO 10993-5 standards. A real-time cell analyzer was used to evaluate cell survival. After seeding 200 µL cell suspensions into the wells of an E-plate 96, HGF and MC3T3-E1 cells were treated with the bioactive components released by the materials and monitored every 15 minutes for 96 hours. Statistical significance was determined using one-way analysis of variance and Tukey-Kramer tests.
Results: After 48 hours of elution, there were significant differences in the HGF cell-index values between the untreated control group and the study groups (P < 0.001) . In addition, the HGF cell-index values of the Leibinger (P < 0.001), Trimed (p < 0.01), and Lorenz (P < 0.01) were significantly lower than that of the untreated control after 72 hours.
When evaluated the after 96 hours, while there were is significant differences among the HGF cell index values of the control and Leibinger (p < 0.01) and Trimed (p < 0.05) groups . There is no differences between the of the control and Lorenz (p>0.05) groups.
Conclusions: Titanium based fixation screws caused a significant decrease in MC3T3-E1 cell viability, there was no adverse effect in all tested materials on human gingival fibroblasts.