IADR Abstract Archives
Therapeutic Effects of Sildenafil on Experimental Mandibular Fractures in Rats: an Immunohistochemical and Histomorphometric Study
Objectives: There is no study about the effect of sildenafil on fracture healing. This study was designed to investigate the effect of sildenafil on fracture healing process.
Methods: Thirtysix female Spraque-Dawley rats (3 month old) were used in this study. Animals were divided into 2 groups randomly, and each group was divided into two as control(C) and study(S) groups. Group C(C1, C2) treated with saline solution and group S(S1,S2) were treated daily with 10mg/kg body weight sildenafil. Histologic, histomorphometric, radiological and immunohistochemical analysis at 1 week and 4 weeks after fracture were performed.
Results: The sildenafil treated group showed an increase in fracture healing scores, with statistically significant (p= 0.00). We observed transition of fibrous callus to cartilage callus and immature bone tissue in the group S1 and increased transition of cartilage callus to completely immature bone callus, in the group S2 which sildenafil had been given. the strong expression of BMP-2 and col-1 was observed in the fibrous matrix and osteoblasts within areas of new bone formation especially in the group S1. The group showed an increase in bone density measurements at 1 week with statistically significant (p= 0.03).
Conclusions: Sildenafil accelerates fracture healing and it can be used as a supporting factor in improvement of fracture healing in various conditions.
Methods: Thirtysix female Spraque-Dawley rats (3 month old) were used in this study. Animals were divided into 2 groups randomly, and each group was divided into two as control(C) and study(S) groups. Group C(C1, C2) treated with saline solution and group S(S1,S2) were treated daily with 10mg/kg body weight sildenafil. Histologic, histomorphometric, radiological and immunohistochemical analysis at 1 week and 4 weeks after fracture were performed.
Results: The sildenafil treated group showed an increase in fracture healing scores, with statistically significant (p= 0.00). We observed transition of fibrous callus to cartilage callus and immature bone tissue in the group S1 and increased transition of cartilage callus to completely immature bone callus, in the group S2 which sildenafil had been given. the strong expression of BMP-2 and col-1 was observed in the fibrous matrix and osteoblasts within areas of new bone formation especially in the group S1. The group showed an increase in bone density measurements at 1 week with statistically significant (p= 0.03).
Conclusions: Sildenafil accelerates fracture healing and it can be used as a supporting factor in improvement of fracture healing in various conditions.