IADR Abstract Archives
IDENTIFICATION OF MICROORGANISMS FROM THE PERIAPEX AREA WITH THE PATIENTS WITH CHRONIC APICAL PERIODONTITIS
Objectives: The aim of this study was to identify microorganisms from periapex lesions with the patients with chronic apical periodontitis after endodontic treatment.
Methods: Microorganisms were isolated from the area of single-rooted teeth periapex after the endodontic treatment.The samples were taken from 10 patients during surgical procedure, apicoectomy. The full thickness flaps were elevated, and the samples were taken by sterile needle from the lesions and drowned in 2 ml liquid media Sheadler broth (Schaedler Broth, Oxoid, United Kingdom). The samples were stored in the transport containers and delivered to the Department of Clinical Microbiology in University Hospital Centre Split. The samples were cultivated simultaneousley for aerobic and anaerobic cultivation 48 hours. Identification of the grown microorganisms was performed using automated commercial system VITEK 2 (bioMerieux, France) with identification cards for single use for each microorganism.
Results: In this research 28 different types of bacteria have been identified from the 10 samples of periapical lesions - 18 (64,3%) gram-positive and 10 (35,7%) gram-negative types. Within the total number of bacterial species there are 8 aerobic bacterial species (28,5%), while the remaining 20 are facultative and strict anaerobes (71,4%). The isolated bacterial species are classified as streptococci (21,4%), staphylococci (21,4%), enterobacteria (10,1%), gram-negative non-ferment bacteria (NF) (17,8%) and anaerobic bacteria (29,3%), with respect to their micromorphology and the conditions needed for their cultivation and growth.
Conclusions: The present study has shown the isolation of 28 microrganisms from a chronical periapical lesions of the single-rooted teeth after endodontic treatment. Anaerobic bacteria were the most prevalent group. From our search of the literature it seems that this might be the first time that Pantoea agglomerans, Oligella ureolytica, Pediococcus pentosaceus and Peptonihilus asaccharolyticus are isolated.
Methods: Microorganisms were isolated from the area of single-rooted teeth periapex after the endodontic treatment.The samples were taken from 10 patients during surgical procedure, apicoectomy. The full thickness flaps were elevated, and the samples were taken by sterile needle from the lesions and drowned in 2 ml liquid media Sheadler broth (Schaedler Broth, Oxoid, United Kingdom). The samples were stored in the transport containers and delivered to the Department of Clinical Microbiology in University Hospital Centre Split. The samples were cultivated simultaneousley for aerobic and anaerobic cultivation 48 hours. Identification of the grown microorganisms was performed using automated commercial system VITEK 2 (bioMerieux, France) with identification cards for single use for each microorganism.
Results: In this research 28 different types of bacteria have been identified from the 10 samples of periapical lesions - 18 (64,3%) gram-positive and 10 (35,7%) gram-negative types. Within the total number of bacterial species there are 8 aerobic bacterial species (28,5%), while the remaining 20 are facultative and strict anaerobes (71,4%). The isolated bacterial species are classified as streptococci (21,4%), staphylococci (21,4%), enterobacteria (10,1%), gram-negative non-ferment bacteria (NF) (17,8%) and anaerobic bacteria (29,3%), with respect to their micromorphology and the conditions needed for their cultivation and growth.
Conclusions: The present study has shown the isolation of 28 microrganisms from a chronical periapical lesions of the single-rooted teeth after endodontic treatment. Anaerobic bacteria were the most prevalent group. From our search of the literature it seems that this might be the first time that Pantoea agglomerans, Oligella ureolytica, Pediococcus pentosaceus and Peptonihilus asaccharolyticus are isolated.