Changes of serum levels of intracelluler enzymes during occlusal trauma

Objectives: The aim of the study was to investigate changes of the serum levels of enzymes such as ALP, AST, ALT, CK, and LDH during experimental occlusal trauma in rabbit.
Methods: 20 New Zealand White male rabbits were selected and divided into two groups. Experimental periodontitis was induced by tying a silk ligature around the neck of mandibulary anterior teeth of rabbits in each group. Clinical indices and radiographic examinations were carried out on experimental days 0, 7, 21 and 90. In group test, experimental occlusal trauma was produced in the animal's lower anterior teeth by insertion of metal crowns, which raised the vertical dimension of occlusion by 2 mm and incorporated interferences into the occlusion. Blood samples were taken 0, 1, 3, 7, 30,or 90 days after the introduction of occlusal trauma, and the serum levels of enzymes such as ALP, AST, ALT, CK, and LDH were measured by ELISA.
Results: At day 3 after insertion of the crowns there was a significant decrease of serum ALP level (p<0.05). Furthermore, there was evidence of trauma from occlusion of the experimental teeth, including a significant increase of tooth mobility, crown fracture, and occlusal wear; radiographically there were signs of breakdown of marginal and interradicular alveolar bone, PDL space widened and root resorption. Statistically significant correlations were not observed between levels of enzymes and periodontal clinical parameters.
Conclusions: Determination of blood levels of enzymes may be appropriate method to evaluate periodontal tissue damage. Analysis of intracellular enzymes seems to be more benefical.