IADR Abstract Archives
The effect of Nd:YAG laser therapy on subgingival human herpesviruses in the treatment of chronic periodontitis
Objectives: The aim of the study was to evaluate the subgingival prevalance of human herpesviruses such as human cytomegalovirus (HCMV), Ebstain-Barr virus (EBV) and herpes simplex virus (HSV) in chronic periodontitis patients before and after Nd:YAG laser treatment.
Methods: Sixty-two patients with untreated chronic periodontitis were randomly assigned in parallel groups to receive SRP alone (control group) or Nd:YAG laser (1064nm, 1.5W, 15Hz, 100mj) followed by SRP (test group). Real-time polymerase chain reaction methods were used to determine the presence of HCMV, EBV, and HSV in crevicular fluid specimens collected at baseline and 3 months after treatment by paper points. Clinical periodontal parameters, including plaque index (PI), bleeding on probing (BOP), probing pocket depth (PPD), probing attachment level (PAL) were assessed at baseline and after 3 months.
Results: The prevalence of HCMV, EBV, and HSV in patients was 34%, 13%, and 9%, respectively. After treatment, the prevalence of HCMV, EBV, and HSV was the lowest in the test group (p<0.05). In the test group, the prevalence of HCMV, EBV, and HSV at following treatment was significantly lower than baseline (p<0.05). Both treatments yielded significant improvements in terms of decrease in PPD and gain in PAL compared to baseline values (p<0.05).
Conclusions: These results showed that Nd:YAG laser therapy provides a much greater reduction the prevalence of HCMV, EBV, and HSV than SRP in periodontitis patients. This study demonstrated that human herpesviruses may occur in periodontitis lesions with relatively high prevalence.
Methods: Sixty-two patients with untreated chronic periodontitis were randomly assigned in parallel groups to receive SRP alone (control group) or Nd:YAG laser (1064nm, 1.5W, 15Hz, 100mj) followed by SRP (test group). Real-time polymerase chain reaction methods were used to determine the presence of HCMV, EBV, and HSV in crevicular fluid specimens collected at baseline and 3 months after treatment by paper points. Clinical periodontal parameters, including plaque index (PI), bleeding on probing (BOP), probing pocket depth (PPD), probing attachment level (PAL) were assessed at baseline and after 3 months.
Results: The prevalence of HCMV, EBV, and HSV in patients was 34%, 13%, and 9%, respectively. After treatment, the prevalence of HCMV, EBV, and HSV was the lowest in the test group (p<0.05). In the test group, the prevalence of HCMV, EBV, and HSV at following treatment was significantly lower than baseline (p<0.05). Both treatments yielded significant improvements in terms of decrease in PPD and gain in PAL compared to baseline values (p<0.05).
Conclusions: These results showed that Nd:YAG laser therapy provides a much greater reduction the prevalence of HCMV, EBV, and HSV than SRP in periodontitis patients. This study demonstrated that human herpesviruses may occur in periodontitis lesions with relatively high prevalence.