IADR Abstract Archives

Antimicrobial Activity of Nanoparticulate Metals against Peri-implantitis Pathogens

Objectives: Plaque accumulation may result in soft tissue inflammation surrounding dental implants and lead to progressive destruction of the supporting tissues. Implant coatings based upon nanoscaled components with osteoconductive and antimicrobial properties are under investigation. Nanoparticulate metals and metal oxides were tested for their activity against bacteria implicated in peri-implant infection and implant failure.

Methods: Nano silver (Ag), cuprous oxide (Cu2O), cupric oxide (CuO), zinc oxide (ZnO), titanium dioxide (Ti2O), tungsten oxide (WO3), Ag+Cu2O blend and Ag+ZnO blend were tested. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values of each nanoparticulate were determined against four species of bacteria (Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans). Time kill assays were carried out to assess the dynamics of the antimicrobial activity.

Results: MIC and MBC values were in the range <100 to 2500 µg/ml and <100 to >2500 µg/ml respectively. Based upon mean values, the activity of the nanoparticulates tested against the 4 species of bacteria was in descending order: Ag > Ag+Cu2O > Cu2O > CuO > Ag+ZnO > ZnO > Ti2O > WO3. Time kill assays with ZnO demonstrated a significant decrease in growth of all species within 4 hours. P. gingivalis showed the greatest sensitivity.

Conclusion: Nanoparticulate metals and metal oxides were shown to have growth inhibitory and bactericidal activity against species of bacteria implicated in peri-implantitis. Nanoparticles, coated onto the surface of titanium using electrohydrodynamic deposition, are currently undergoing assessment as regards antimicrobial properties.

Work supported by an IADR / GSK Innovation in Oral Care Award.


British Division Meeting
2011 British Division Meeting (Sheffield, England)
Sheffield, England
2011
121
Scientific Groups
  • Vargas-reus, Miguel  ( Queen Mary, University of London, London, N/A, United Kingdom )
  • Memarzadeh, Kaveh  ( Queen Mary, University of London, London, N/A, United Kingdom )
  • Huang, Jie  ( University College London, London, N/A, United Kingdom )
  • Ren, Guogang  ( University of Hertfordshire, Hatfield, N/A, United Kingdom )
  • Allaker, Robert  ( Queen Mary, University of London, London, N/A, United Kingdom )
    • Poster Session
    Posters: Wednesday
    09/14/2011