IADR Abstract Archives
In vivo Expression of Site Specific Chemokines in Oral Mucosa
Background:
Lymphocytes show a restricted pattern of tissue recirculation and it has been postulated that in skin, small intestine and salivary glands this is controlled by site-specific chemokines and lymphocyte homing receptors. We have shown previously that oral keratinocytes express skin (CCL27), small intestine (CCL25) and salivary gland (CCL28) associated chemokines in vitro suggesting the concept of site specific chemokines may be an oversimplification.
Objectives:
To determine expression of CCL25, CCL27 & CCL28 in oral mucosa in vivo.
Methods: Immunohistochemistry: Ten cases each: normal (NS) and inflamed skin (IS), normal oral mucosa (NOM), lichen planus (LP), chronic hyperplastic candidiasis (CHC), oral Crohn's disease (OCD) & oral cancer (OSCC) were stained for CCL25, CCL27 & CCL28. Epithelial expression was quantified using an ACIS III scanner and the results shown as expression index (EI= % of +ve cells x staining intensity).
Results:
Chemokine
| CCL25
| CCL27
| CCL28
|
Tissue
| EI
| EI
| EI
|
NOM | 2845(±619.7)
| 1674.7(±499.6)
| 1424.9(±536.5)
|
LP | 6646(±746.8)*
| 6395.1(±412.6)*
| 3732.6(±917.7)*
|
CHC | 8339(±295.4)*
| 7187.5(±521.0)*
| 8458.5(±675.7)*
|
OCD | 159(±74.3)*
| 761.4(±493.1)
| 159.2(±90.3)
|
OSCC | 5454(±608.4)*
| 6075.7(±598.1)*
| 2918.6(±1313.6)
|
NS | 1743(±439.4)
| 1535.6(±191.6)
| 1311.2(±570.9)
|
IS | 5415(±478.0)**
| 4529.6(±374.8)**
| 3276.6(±509.4)**
|
* (P = <0.05) compared to NM.,
** (P=<0.05) compared to NS
CCL25, 27, 28 are all significantly upregulated in CHC and LP compared to NOM and in IS compared with NS. In contrast CCL25 was downregulated in OCD compared to NOM but only CCL25 and CCL27, not CCL28, were upregulated in OSCC compared with NOM.
Conclusion:
Multiple chemokines are expressed in the skin & oral mucosa & the expression pattern observed in a range of inflammatory diseases confirms our in vitro findings. The underlying cause for such changes in inflammatory tissues is not clear but our results suggest that the site-specific expression of chemokines in normal tissue is altered in inflammation.
Funded by King Abdulaziz University (KAU), Jeddah, Saudi Arabia