IADR Abstract Archives
Chemical Speciation Analysis of Influence of Parotid Saliva on Demineralisation
Objectives: Parotid saliva (PS) contributes over 50% of salivary secretion under stimulated conditions and contains inorganic components in single ion and complex ion form both of which influence the chemistry of caries process. This study aimed to correlate the degree of saturation with respect to various calcium phosphates of PS inorganic components with caries scores in healthy adults.
Methods: Stimulated PS was collected from 21 adults using a Lashley cup. Caries scores were classified using the International Caries Detection and Assessment System (ICDAS). The PS flow rate and pH were recorded. Total concentrations of inorganic elements (K+, Pi, Na+, Ca2+, Mg2+, Al3+, Sr2+, Zi2+, Li+) were measured using Inductively Coupled Plasma-Optical Emission Spectrometer (ICP-OES). The activity of each inorganic element was calculated by chemical speciation calculations. Pearson’s correlation coefficients (r) were calculated to determine bivariate relationship and statistical significance (p<0.05).
Results: PS flow-rates were 0.07-0.56 ml/min, pH 5.9-7.6, and ICDAS scores 0.03-2.48. The PS pH was significantly correlated with the caries score (r -0.536; p<0.05); whereas the flow rate was not. A statistically significant correlation was found between caries score and PS activity of bicarbonate ions {HCO3-} (r -0.495, p<0.05), hydrogen phosphate ions {HPO42-} (r 0.806; p<0.05), and dihydrogen phosphate ions {H2PO4-} (r 0.438; p<0.05). The activity of calcium hydrogen carbonate ions {CaHCO3+} was negatively correlated with subjects’ caries score, however the correlation was not statistically significant. The activities of ionised calcium {Ca2+} and aluminium {Al3+} were statistically significantly correlated with the caries score (r 0.425, p<0.05; and r -0.423, p<0.05) and with the pH of PS (r -0.426, p<0.05; and r 0.852, p<0.05).
Conclusions: The analysis of PS identified inorganic components involved in the caries process. Chemical species calculations of PS have the potential to improve approaches for clinical caries prevention.
Methods: Stimulated PS was collected from 21 adults using a Lashley cup. Caries scores were classified using the International Caries Detection and Assessment System (ICDAS). The PS flow rate and pH were recorded. Total concentrations of inorganic elements (K+, Pi, Na+, Ca2+, Mg2+, Al3+, Sr2+, Zi2+, Li+) were measured using Inductively Coupled Plasma-Optical Emission Spectrometer (ICP-OES). The activity of each inorganic element was calculated by chemical speciation calculations. Pearson’s correlation coefficients (r) were calculated to determine bivariate relationship and statistical significance (p<0.05).
Results: PS flow-rates were 0.07-0.56 ml/min, pH 5.9-7.6, and ICDAS scores 0.03-2.48. The PS pH was significantly correlated with the caries score (r -0.536; p<0.05); whereas the flow rate was not. A statistically significant correlation was found between caries score and PS activity of bicarbonate ions {HCO3-} (r -0.495, p<0.05), hydrogen phosphate ions {HPO42-} (r 0.806; p<0.05), and dihydrogen phosphate ions {H2PO4-} (r 0.438; p<0.05). The activity of calcium hydrogen carbonate ions {CaHCO3+} was negatively correlated with subjects’ caries score, however the correlation was not statistically significant. The activities of ionised calcium {Ca2+} and aluminium {Al3+} were statistically significantly correlated with the caries score (r 0.425, p<0.05; and r -0.423, p<0.05) and with the pH of PS (r -0.426, p<0.05; and r 0.852, p<0.05).
Conclusions: The analysis of PS identified inorganic components involved in the caries process. Chemical species calculations of PS have the potential to improve approaches for clinical caries prevention.