IADR Abstract Archives
Biomimetic Self-Assembling Peptides as a Treatment for Hypomineralised Dental Enamel
Objectives: Molar incisor hypomineralisation (MIH) is a developmental defect, which causes low mineral density and misaligned crystallites in dental enamel. A biomimetic self-assembling peptide (SAP) P11-4 is able to direct de novo crystallite nucleation in enamel affected by demineralisation, therefore it has the potential to guide oriented, and aligned mineral growth in hypomineralised enamel. This study aims to assess the possibility of using P11-4 as a novel treatment of hypomineralised enamel caused by MIH by characterising the crystallographic alignment in MIH enamel with or without SAP treatment and correlating this to mineral density.
Methods: Five human first permanent molars moderately affected with MIH were selected according to the diagnostic criteria of Mathu-Muju and Wright (2006). Teeth were sectioned and the buccal segments were used to create enamel slabs of the normal enamel (NE) and hypomineralised enamel (HE); each sample was cut in half through the mid-line of the MIH lesion. One-half was used as a baseline assessment and the other half for the SAPs application. All samples were then mapped using 2D synchrotron X-ray diffraction (2D- SXRD) and micro-computed tomography (micro CT).
Results: Preliminary results of 2D- SXRD revealed coexistence of two crystallite populations, with higher percentage of contribution of one orientation population, and overall low texture magnitude of hypomineralised enamel structure. Following treatment by P11-4, data have shown increased texture magnitude in both populations within the full thickness of enamel. Correspondingly, the initial micro CT analysis have shown statistically significant increase in mineral density in hypomineralised enamel after SAPs treatment (p > .05) while there was no significant change in mineral density in normal enamel samples (p < .05).
Conclusions: Biomimetic self-assembling peptides have shown the potential to promote oriented crystallite nucleation and growth for the first time in hypomineralised enamel.
Methods: Five human first permanent molars moderately affected with MIH were selected according to the diagnostic criteria of Mathu-Muju and Wright (2006). Teeth were sectioned and the buccal segments were used to create enamel slabs of the normal enamel (NE) and hypomineralised enamel (HE); each sample was cut in half through the mid-line of the MIH lesion. One-half was used as a baseline assessment and the other half for the SAPs application. All samples were then mapped using 2D synchrotron X-ray diffraction (2D- SXRD) and micro-computed tomography (micro CT).
Results: Preliminary results of 2D- SXRD revealed coexistence of two crystallite populations, with higher percentage of contribution of one orientation population, and overall low texture magnitude of hypomineralised enamel structure. Following treatment by P11-4, data have shown increased texture magnitude in both populations within the full thickness of enamel. Correspondingly, the initial micro CT analysis have shown statistically significant increase in mineral density in hypomineralised enamel after SAPs treatment (p > .05) while there was no significant change in mineral density in normal enamel samples (p < .05).
Conclusions: Biomimetic self-assembling peptides have shown the potential to promote oriented crystallite nucleation and growth for the first time in hypomineralised enamel.