IADR Abstract Archives
Assessment of Different Toothpastes on Root Caries Using X-ray Diffraction
Objectives: To assess the structural characteristics of artificial root caries (ARC) following the use of toothpaste either containing 5000 ppm or 540 ppm fluoride with bioglass.
Methods: The crowns of 23 extracted sound teeth were cut by leaving the root surfaces and divided into four pieces (n=84). 70 samples were used to develop ARC using a demineralisation solution (pH 4.8) for a period of five days, whilst 14 samples were left untreated as baseline. The pH cycling including the demin-remin solution (pH 4.8 and pH 7) for 13 days. 56 out of 70 samples with ARC received toothbrushing twice daily with assigned toothpastes. These samples were randomly allocated into six groups (n=14 for each group). Group 1. No ARC treatment, Group 2. ARC treatment alone, Group 3. Bioglass with 540 ppm CaF, Group 4. 5,000 NaF, Group 5. 1,450 ppm NaF, and Group 6. Deionised water. X-ray diffraction (XRD) was carried out to detect apatite formation.
Results: The XRD pattern revealed strong diffraction lines at 26.0°, 31.9° 2θ for the Group 1. These peaks were representative of hydroxyapatite. Group 2 exhibited a substantial decrease in these diffraction line and loss of 41% mineral intensity compared to Group 1. The diffraction lines at 26.0°, 31.9° and 32.9° 2θ reappeared following the use of assigned toothpastes confirming the evidence of remineralisation on root dentine in Groups 3, 4 and 5, however there was loss of 33%, 39% and 38% mineral intensity respectively when compared to Group 1. There was lack of prominent peaks with a loss of 49% mineral intensity in Group 6.
Conclusions: This laboratory-based study provided an evidence that using different toothpastes can potentially promote remineralisation on ARC. Toothpaste containing 540 ppm CaF with bioglass showed less mineral intensity loss compared to toothpastes containing 5,000 ppm and 1,450 ppm NaF.
Methods: The crowns of 23 extracted sound teeth were cut by leaving the root surfaces and divided into four pieces (n=84). 70 samples were used to develop ARC using a demineralisation solution (pH 4.8) for a period of five days, whilst 14 samples were left untreated as baseline. The pH cycling including the demin-remin solution (pH 4.8 and pH 7) for 13 days. 56 out of 70 samples with ARC received toothbrushing twice daily with assigned toothpastes. These samples were randomly allocated into six groups (n=14 for each group). Group 1. No ARC treatment, Group 2. ARC treatment alone, Group 3. Bioglass with 540 ppm CaF, Group 4. 5,000 NaF, Group 5. 1,450 ppm NaF, and Group 6. Deionised water. X-ray diffraction (XRD) was carried out to detect apatite formation.
Results: The XRD pattern revealed strong diffraction lines at 26.0°, 31.9° 2θ for the Group 1. These peaks were representative of hydroxyapatite. Group 2 exhibited a substantial decrease in these diffraction line and loss of 41% mineral intensity compared to Group 1. The diffraction lines at 26.0°, 31.9° and 32.9° 2θ reappeared following the use of assigned toothpastes confirming the evidence of remineralisation on root dentine in Groups 3, 4 and 5, however there was loss of 33%, 39% and 38% mineral intensity respectively when compared to Group 1. There was lack of prominent peaks with a loss of 49% mineral intensity in Group 6.
Conclusions: This laboratory-based study provided an evidence that using different toothpastes can potentially promote remineralisation on ARC. Toothpaste containing 540 ppm CaF with bioglass showed less mineral intensity loss compared to toothpastes containing 5,000 ppm and 1,450 ppm NaF.