IADR Abstract Archives
Impact of Hyperglycaemia and Hyperkalaemia on Fusobacterium Nucleatum Polymorphum Behaviour
Objectives: To investigate the effect of gingival crevicular fluid (GCF) glucose and potassium levels (physiological, periodontitis and diabetes relevant) on the growth and haemolysins activity of Fusobacterium nucleatum spp polymorphum (FNP) ( ATCC10953) in vitro.
Methods: Yeast peptone broth media (no glucose), was supplemented with range of glucose concentrations (0, 5, 10, 17, 25, 33mM) mimicking levels present in GCF, in health (5, 10mM) and diabetes (17, 23, 33mM). In addition, yeast peptone broth media (no potassium) was supplemented with a range of potassium concentrations (0, 4, 8, 12, 24, 70 mEq/l) relevant to those present in GCF in healthy periodontium, periodontitis and diabetes. FNP (ATCC10953) was grown in these media and bacterial counts were performed by measuring optical density of the samples over 6 days.
Haemolysins activity was determined using haemolysis assay for FNP was grown in culture media supplemented either with different glucose or potassium levels in health and disease then incubated with 5% or 25% sheep red blood cells. Increase in optical density among the bacterial samples, illustrated as haemoglobin was released due to the secretion of haemolysins.
Results: Data indicated a statistically significant dose dependent enhancement in FNP growth with increasing levels of glucose levels. No significant changes in growth were seen with the range of potassium concentrations tested. Neither glucose nor potassium supplementation enhanced FNP haemolysins activity, in the presence of sheep red blood cells.
Conclusions: This study showed that glucose levels that are consistent with hyperglycaemic conditions seen in GCF enhanced FNP growth, this could suggest a potential mechanism that contributes to the strong association between diabetes and periodontitis.
While no effect of hyperglycaemia or hyperkalaemia on haemolysis was found, further investigations are needed on human red blood cells to confirm the results.
Methods: Yeast peptone broth media (no glucose), was supplemented with range of glucose concentrations (0, 5, 10, 17, 25, 33mM) mimicking levels present in GCF, in health (5, 10mM) and diabetes (17, 23, 33mM). In addition, yeast peptone broth media (no potassium) was supplemented with a range of potassium concentrations (0, 4, 8, 12, 24, 70 mEq/l) relevant to those present in GCF in healthy periodontium, periodontitis and diabetes. FNP (ATCC10953) was grown in these media and bacterial counts were performed by measuring optical density of the samples over 6 days.
Haemolysins activity was determined using haemolysis assay for FNP was grown in culture media supplemented either with different glucose or potassium levels in health and disease then incubated with 5% or 25% sheep red blood cells. Increase in optical density among the bacterial samples, illustrated as haemoglobin was released due to the secretion of haemolysins.
Results: Data indicated a statistically significant dose dependent enhancement in FNP growth with increasing levels of glucose levels. No significant changes in growth were seen with the range of potassium concentrations tested. Neither glucose nor potassium supplementation enhanced FNP haemolysins activity, in the presence of sheep red blood cells.
Conclusions: This study showed that glucose levels that are consistent with hyperglycaemic conditions seen in GCF enhanced FNP growth, this could suggest a potential mechanism that contributes to the strong association between diabetes and periodontitis.
While no effect of hyperglycaemia or hyperkalaemia on haemolysis was found, further investigations are needed on human red blood cells to confirm the results.