IADR Abstract Archives
Epigenetic Regulation in Peri-Implantitis Correlates With Disease Severity
Objectives: DNA methylation is an epigenetic effect which can alter the activity of the genome without affecting the DNA sequence. Therefore, this may be a relevant regulator of inflammatory and destructive processes underlying a peri-implant disease. We used DNA methylation arrays to investigate methylation patterns of tissues obtained from peri-implantitis sites.
Methods: We obtained a total of 47 tissue samples of untreated peri-implantitis sites [probing depths (PD) ≥ 5mm, positive bleeding on probing (BoP), radiological bone loss ≥ 3mm] from 30 systemically healthy non-smoking individuals [mean PD=8.04mm, range 5-11mm]. DNA-methylation was investigated using Illumina Infinium MethylationEPIC BeadChips and the IlluminaEPIC (850K) platform. Pre-processing, normalization, and subsequent differential methylation in correlation with disease severity analysis was conducted on a R platform utilizing established R-packages (e.g., limma, minfi, DMRcate, missMethyl). Potential confounding factors, such as age, gender, multiple testing per individual were addressed in our regression analysis design.
Results: A total of 121500 differentially methylated positions (DMPs) in correlation with disease severity were identified, corresponding to 6562 differentially methylated regions (DMRs) considering a threshold of FDR<0.05. The analysis of genes correlating with differentially methylated CpGs exhibited over-representation of a variety of pathways including those related to MAPK signalling, osteoclast differentiation and choline metabolism in cancer.
Conclusions: Severity of peri-implantitis is correlated with significant differences in DNA methylation signatures. This is an indicator for epigenetic regulatory pathways in this inflammatory disease. Further studies of DNA methylation, other epigenetic regulatory processes and gene expression may clarify underlying pathophysiological processes in more depth.
Methods: We obtained a total of 47 tissue samples of untreated peri-implantitis sites [probing depths (PD) ≥ 5mm, positive bleeding on probing (BoP), radiological bone loss ≥ 3mm] from 30 systemically healthy non-smoking individuals [mean PD=8.04mm, range 5-11mm]. DNA-methylation was investigated using Illumina Infinium MethylationEPIC BeadChips and the IlluminaEPIC (850K) platform. Pre-processing, normalization, and subsequent differential methylation in correlation with disease severity analysis was conducted on a R platform utilizing established R-packages (e.g., limma, minfi, DMRcate, missMethyl). Potential confounding factors, such as age, gender, multiple testing per individual were addressed in our regression analysis design.
Results: A total of 121500 differentially methylated positions (DMPs) in correlation with disease severity were identified, corresponding to 6562 differentially methylated regions (DMRs) considering a threshold of FDR<0.05. The analysis of genes correlating with differentially methylated CpGs exhibited over-representation of a variety of pathways including those related to MAPK signalling, osteoclast differentiation and choline metabolism in cancer.
Conclusions: Severity of peri-implantitis is correlated with significant differences in DNA methylation signatures. This is an indicator for epigenetic regulatory pathways in this inflammatory disease. Further studies of DNA methylation, other epigenetic regulatory processes and gene expression may clarify underlying pathophysiological processes in more depth.