IADR Abstract Archives
The Effect of Diabetes on Osteogenic Differentiation of MSCs
Objectives: This study aims to investigate the effect of type 2 diabetes mellitus on proliferation rate, clonogenicity and osteogenic differentiation potentials of bone marrow mesenchymal stromal cells (BM-MSCs).
Methods: BM-MSCs were isolated from osteoarthritic knee joints of type 2 diabetic and non-diabetic donors using collagenase digestion method. Cultured cells were compared for their proliferative capacities using colony forming unit (CFU) and population doubling time (PDT) assays. The osteogenic potentials of BM-MSCs were investigated under osteogenic conditions (OC) and compared for calcium deposition using Alizarin Red staining and quantification and also alkaline phosphatase staining after 1, 2 and 3 weeks in culture.
Results: Diabetic BM-MSCs in average contained 16% fewer CFUs compared to non-diabetic cells at passages 1-2 and the median PDT of diabetic cells was 20% higher than that of non-diabetic BM-MSCs up to passage 5.
Diabetic BM-MSCs calcification at all time points under OC was on average 39% of that shown by non-diabetic cells. The intensity of alkaline phosphatase staining of both diabetic and non-diabetic cells showed subtle increase in OC vs basal conditions and through different time points which correlated with Alizarin Red staining intensity.
Conclusions: These preliminary results show that osteoarthritic BM-MSCs from type 2 diabetic patients have weaker proliferative and osteogenic capacities compared to osteoarthritic non diabetic cells. This work can have future clinical implications for autologous stem cells based regenerative therapies in type 2 diabetics including periodontal regeneration.
Methods: BM-MSCs were isolated from osteoarthritic knee joints of type 2 diabetic and non-diabetic donors using collagenase digestion method. Cultured cells were compared for their proliferative capacities using colony forming unit (CFU) and population doubling time (PDT) assays. The osteogenic potentials of BM-MSCs were investigated under osteogenic conditions (OC) and compared for calcium deposition using Alizarin Red staining and quantification and also alkaline phosphatase staining after 1, 2 and 3 weeks in culture.
Results: Diabetic BM-MSCs in average contained 16% fewer CFUs compared to non-diabetic cells at passages 1-2 and the median PDT of diabetic cells was 20% higher than that of non-diabetic BM-MSCs up to passage 5.
Diabetic BM-MSCs calcification at all time points under OC was on average 39% of that shown by non-diabetic cells. The intensity of alkaline phosphatase staining of both diabetic and non-diabetic cells showed subtle increase in OC vs basal conditions and through different time points which correlated with Alizarin Red staining intensity.
Conclusions: These preliminary results show that osteoarthritic BM-MSCs from type 2 diabetic patients have weaker proliferative and osteogenic capacities compared to osteoarthritic non diabetic cells. This work can have future clinical implications for autologous stem cells based regenerative therapies in type 2 diabetics including periodontal regeneration.