IADR Abstract Archives
Identifying Internal Changes in the Teeth in Response to External Challenges Using X-ray Microtomography
Objectives: To study the defense mechanism in teeth using X-ray Microtomography which is sensitive to detect any mineral changes
Whitelockite (Tricalcium phosphate) with traces of magnesium in cracks.
Raman Spectroscopy, Scanning electron microscopy (RDX-SEM) and synchrotron X-ray diffraction will also be used to study the reactive mineral responses.
Methods: The collection of multiple samples of extracted teeth, subjected to a variety of dental insults prior to extraction from patients of different age groups and scan these teeth with high contrast X-ray microtomography (XMT or micro-CT) to identify the paths of hypermineralization within the dentine. In addition to XMT, SEM, and synchrotron X-ray diffraction is used to determine the nature of the formed mineral. Although the mechanism for reactive and reparative dentine formation is understood, preliminary studies have suggested that mineral transport through the dentine tubules may also have a protective effect.
Results: Pulp responds to any damage done to the surface of the tooth and it is very clearly evident from the XMT images in the form of patterns of hypermineralization. From initial results, It appears that mineral originates in the pulp and propagates through the dentine tubules towards the enamel, forming peritubular dentine (Tri-calcium phosphate - magnesium substituted) in the process, and possibly contributing to remineralization or mineral formation within enamel cracks that could reduce or inhibit crack-propagation. This was further investigated using Raman Spectroscopy and SEM -XRD that what are the minerals formed in between the cracks.
This is based on anonymized teeth, we cannot rule out the hypothesis that is related to age, gender, and ethnicity at the time of extraction.
Conclusions: In addition to XMT, SEM will be used to compare the surfaces of the tubule and the site of mineral formed and synchrotron X-ray diffraction will be used to determine the nature of the mineral. Collecting a detailed dental history prior to extraction using anonymized data, is beneficial in determining the responses from any previous dental insult. Increasing the current understanding of the natural defenses will, in turn, enable therapeutic developments to take advantage of these processes.
Whitelockite (Tricalcium phosphate) with traces of magnesium in cracks.
Raman Spectroscopy, Scanning electron microscopy (RDX-SEM) and synchrotron X-ray diffraction will also be used to study the reactive mineral responses.
Methods: The collection of multiple samples of extracted teeth, subjected to a variety of dental insults prior to extraction from patients of different age groups and scan these teeth with high contrast X-ray microtomography (XMT or micro-CT) to identify the paths of hypermineralization within the dentine. In addition to XMT, SEM, and synchrotron X-ray diffraction is used to determine the nature of the formed mineral. Although the mechanism for reactive and reparative dentine formation is understood, preliminary studies have suggested that mineral transport through the dentine tubules may also have a protective effect.
Results: Pulp responds to any damage done to the surface of the tooth and it is very clearly evident from the XMT images in the form of patterns of hypermineralization. From initial results, It appears that mineral originates in the pulp and propagates through the dentine tubules towards the enamel, forming peritubular dentine (Tri-calcium phosphate - magnesium substituted) in the process, and possibly contributing to remineralization or mineral formation within enamel cracks that could reduce or inhibit crack-propagation. This was further investigated using Raman Spectroscopy and SEM -XRD that what are the minerals formed in between the cracks.
This is based on anonymized teeth, we cannot rule out the hypothesis that is related to age, gender, and ethnicity at the time of extraction.
Conclusions: In addition to XMT, SEM will be used to compare the surfaces of the tubule and the site of mineral formed and synchrotron X-ray diffraction will be used to determine the nature of the mineral. Collecting a detailed dental history prior to extraction using anonymized data, is beneficial in determining the responses from any previous dental insult. Increasing the current understanding of the natural defenses will, in turn, enable therapeutic developments to take advantage of these processes.