IADR Abstract Archives
in Vivo Evaluation of Cotton-Wool-Like Bioactive Glasses for Bone Regeneration
Objectives: Histomorphometric and microCT investigation of the effect of cotton-wool-like bioactive glasses (Biowool) on bone healing in rat tibial defects.
Methods: Biowool was synthesised using a combination of sol-gel process with electro-spinning technique. In this investigation, 27 rats were used and randomly distributed into 3 groups: Biowool group (Group I), Bioglass® group (Group II) and control group (Group III, bone defect without treatment). Three animals from each group was euthanized at 2, 6 and 12 weeks post-surgery. Bone healing was evaluated histologically using H and E staining and radiographically using micro-CT and scanning electron microscopy (SEM).
Results: At 2 weeks, new trabecular bone was present in groups I and II in histological and radiographical images and some areas presented a loosely arranged connective tissue with inter-fibre spaces in histological images. At 6th week, histological and micro-CT results showed more bone formation with linear cortical bone but still some remaining large medullary spaces in groups I and II, however, histological images of group I showed remarkable presence of blood vessels in the central area of the bone morrow. At 12th week, histological and radiographical results revealed complete cortical bone formation in groups I and II but with large medullar spaces in particular for group II. The amount of bone formed in the group III in histological and micro-CT images was similar to those observed in groups I and II.
Conclusions: The cotton-wool-like bioactive glass induced bone formation similar to Bioglass®, with evidence for mature blood vessel formation. Thus, the cotton-wool-like bioactive glasses could promote tissue repair when used as bone graft in tooth extraction sockets.
Methods: Biowool was synthesised using a combination of sol-gel process with electro-spinning technique. In this investigation, 27 rats were used and randomly distributed into 3 groups: Biowool group (Group I), Bioglass® group (Group II) and control group (Group III, bone defect without treatment). Three animals from each group was euthanized at 2, 6 and 12 weeks post-surgery. Bone healing was evaluated histologically using H and E staining and radiographically using micro-CT and scanning electron microscopy (SEM).
Results: At 2 weeks, new trabecular bone was present in groups I and II in histological and radiographical images and some areas presented a loosely arranged connective tissue with inter-fibre spaces in histological images. At 6th week, histological and micro-CT results showed more bone formation with linear cortical bone but still some remaining large medullary spaces in groups I and II, however, histological images of group I showed remarkable presence of blood vessels in the central area of the bone morrow. At 12th week, histological and radiographical results revealed complete cortical bone formation in groups I and II but with large medullar spaces in particular for group II. The amount of bone formed in the group III in histological and micro-CT images was similar to those observed in groups I and II.
Conclusions: The cotton-wool-like bioactive glass induced bone formation similar to Bioglass®, with evidence for mature blood vessel formation. Thus, the cotton-wool-like bioactive glasses could promote tissue repair when used as bone graft in tooth extraction sockets.