IADR Abstract Archives
How do Ti Based Nanoparticles Alter Chemoattractant Properties of Chemokines?
Objectives: This study aimed to determine the effects of Ti based nanoparticles on known chemoattractant chemokines. It is known that implanted Ti devices produce wear particles within tissues of varying sizes and compositions, from nanoscale upwards. When present in tissues adjacent to implanted Ti devices, such as dental implants, these particles may affect immune or inflammatory responses occurring in response to the presence of bacterial biofilms or other inflammatory stimuli. The hypothesis is that the presence of Ti species within tissues can affect the inflammatory response within tissues by binding to and inactivating key chemokines in the inflammatory and immune response.
Methods: Freshly isolated neutrophils from healthy volunteers were exposed to a panel of chemoattractant chemokines (e.g. CXCL8) in the presence and absence of a variety of Ti (e.g. anatsase, rutile, mixed) based nanoparticles at differing concentrations. The chemotaxis velocity and directionality of neutrophils was measured.
Results: The chemoattractant properties of CXCL8 are negatively affected in a dose dependent manner by the presence of TiO2 nanoparticles in anatase form. The parameters measured were speed, velocity and chemotactic index.
Conclusions: The presence of Ti species within tissues has the potential to adversely affect the chemoattractant properties of important chemokines. Further research to characterise the nature of these interactions is required and other potential Ti product – chemokine interactions, as the potential impact on the progression of inflammatory conditions such as peri-implantitis may be clinically important.
Methods: Freshly isolated neutrophils from healthy volunteers were exposed to a panel of chemoattractant chemokines (e.g. CXCL8) in the presence and absence of a variety of Ti (e.g. anatsase, rutile, mixed) based nanoparticles at differing concentrations. The chemotaxis velocity and directionality of neutrophils was measured.
Results: The chemoattractant properties of CXCL8 are negatively affected in a dose dependent manner by the presence of TiO2 nanoparticles in anatase form. The parameters measured were speed, velocity and chemotactic index.
Conclusions: The presence of Ti species within tissues has the potential to adversely affect the chemoattractant properties of important chemokines. Further research to characterise the nature of these interactions is required and other potential Ti product – chemokine interactions, as the potential impact on the progression of inflammatory conditions such as peri-implantitis may be clinically important.