IADR Abstract Archives
Biocompatibility of Silver Nanoparticles Gel as an Endodontic Intra-Canal Medicament
Objectives: This study aimed to evaluate the effects of a synthesized silver nanoparticles (AgNPs) gel with different concentrations as an intra-canal medicament for regenerative endodontics on viability, attachment and proliferation of dental pulp stromal cells (DPSCs).
Methods: Ninety-five standardized dentin discs (4x4x1 mm) were prepared from extracted human single-rooted teeth for periodontal reasons following institutional ethical approval. Samples were cleaned, autoclaved, and treated with: 1.5%NaOCl, Saline and 17% EDTA then randomly assigned into 5 groups that received 50µl of: 0.01%AgNPs, 0.015%AgNPs, 0.02%AgNPs, Calcium hydroxide (Ca(OH)2) or no treatment for 1 week. Discs were washed with Saline and 17%EDTA then seeded with DPSCs (1x104 cells/well) in non-treated low attachment 24-well culture plates. Seeded dentin discs were incubated for 3 and 7 days, media was collected after 24h of seeding for LDH assays and to count unattached cells. At each time point cytotoxicity (LDH assay), cell viability (live/dead staining with Confocal microscopy), and cell proliferation (WST1 assay) were assessed. Statistical analysis was done using One-Way ANOVA followed by post-hoc comparisons with Bonferroni correction.
Results: After 24 hours, DPSCs attachment percentage ranged between 92.66% ±4.54 and 95.08% ±1.44 without significant difference between groups (P=0.126). Cell viability was ≥92% at 24 hours for all groups which barely dropped to less than 60% at 3 days then started to rise again at 7 days. There was no significant difference at any-time point except for 0.01%AgNPs which had the highest cytotoxicity of 7.26% ±10.89, 40.32% ±2.92 and 32.11% ±2.55 at 24hrs, 3 and 7 days, respectively. DPSCs proliferation increased significantly from 3 to 7 days except for Ca(OH)2 which showed 45.89% ±12.12 and 79.25% ±51.68 proliferation percentage at 3 and 7 days, respectively.
Conclusions: AgNPs gel showed comparable biocompatibility to Ca(OH)2 when used as an intra-canal medicament however, its effects may be concentration-dependent warranting further investigation.
Methods: Ninety-five standardized dentin discs (4x4x1 mm) were prepared from extracted human single-rooted teeth for periodontal reasons following institutional ethical approval. Samples were cleaned, autoclaved, and treated with: 1.5%NaOCl, Saline and 17% EDTA then randomly assigned into 5 groups that received 50µl of: 0.01%AgNPs, 0.015%AgNPs, 0.02%AgNPs, Calcium hydroxide (Ca(OH)2) or no treatment for 1 week. Discs were washed with Saline and 17%EDTA then seeded with DPSCs (1x104 cells/well) in non-treated low attachment 24-well culture plates. Seeded dentin discs were incubated for 3 and 7 days, media was collected after 24h of seeding for LDH assays and to count unattached cells. At each time point cytotoxicity (LDH assay), cell viability (live/dead staining with Confocal microscopy), and cell proliferation (WST1 assay) were assessed. Statistical analysis was done using One-Way ANOVA followed by post-hoc comparisons with Bonferroni correction.
Results: After 24 hours, DPSCs attachment percentage ranged between 92.66% ±4.54 and 95.08% ±1.44 without significant difference between groups (P=0.126). Cell viability was ≥92% at 24 hours for all groups which barely dropped to less than 60% at 3 days then started to rise again at 7 days. There was no significant difference at any-time point except for 0.01%AgNPs which had the highest cytotoxicity of 7.26% ±10.89, 40.32% ±2.92 and 32.11% ±2.55 at 24hrs, 3 and 7 days, respectively. DPSCs proliferation increased significantly from 3 to 7 days except for Ca(OH)2 which showed 45.89% ±12.12 and 79.25% ±51.68 proliferation percentage at 3 and 7 days, respectively.
Conclusions: AgNPs gel showed comparable biocompatibility to Ca(OH)2 when used as an intra-canal medicament however, its effects may be concentration-dependent warranting further investigation.