IADR Abstract Archives
Expression of periodontal ligament-associated markers in oral and extra-oral mesechymal stem cells
Objectives: Heterogenity of transcriptomic and proteomic profiles exists within mesenchymal stem cells (MSCs) dependent upon anatomical source. We have previously shown that periodontal ligament (PDL) MSCs express distinct gene expression profiles of PDL-associated marker proteins to those derived from bone marrow (BM) both constitutively and when driven to differentiate. Here we investigated the expression of these markers in dental pulp stem cells (DPSCs) to compare profiles with PDL and BM-derived MSCs.
Methods: Primary human PDL, dental and BM-derived MSCs from different individuals were expanded in vitro and then cultured for periods of up to 21 days in normal and osteogenic media. Expression of osteogenic, adipogenic and PDL-associated genes was tested by qRT-PCR at time points until 21 days of differentiation.
Results: No statistically significant differences were found in expression levels of the osteogenic and adipogenic marker genes, Runx2 and PPARgamma, between PDL MSCs and DPSCs. Expression levels of the PDL-associated marker gene, PLAP-1, was significantly higher in both PDL MSCs and DPSCs than in BMSCs, however there were no differences between PDL MSCs and DPSCs. Expression of nestin and periostin was not significantly different between any of the three MSC types.
Conclusions: MSCs derived from different sources showed similar osteogenic and adipogenic expression profiles both constitutively and during differentiation, however the selective expression of PLAP-1 in dental derived MSCs indicates inherent differences dependent upon origin.
Methods: Primary human PDL, dental and BM-derived MSCs from different individuals were expanded in vitro and then cultured for periods of up to 21 days in normal and osteogenic media. Expression of osteogenic, adipogenic and PDL-associated genes was tested by qRT-PCR at time points until 21 days of differentiation.
Results: No statistically significant differences were found in expression levels of the osteogenic and adipogenic marker genes, Runx2 and PPARgamma, between PDL MSCs and DPSCs. Expression levels of the PDL-associated marker gene, PLAP-1, was significantly higher in both PDL MSCs and DPSCs than in BMSCs, however there were no differences between PDL MSCs and DPSCs. Expression of nestin and periostin was not significantly different between any of the three MSC types.
Conclusions: MSCs derived from different sources showed similar osteogenic and adipogenic expression profiles both constitutively and during differentiation, however the selective expression of PLAP-1 in dental derived MSCs indicates inherent differences dependent upon origin.