IADR Abstract Archives
Protection Effects of Bioglass® and Pro-Argin® Layers Formed On Dentine
Objectives: Exposed dentine in the oral environment is at risk from mechanical damage due to brushing or mastication as well as chemical dissolution due to acidic food or bacterial attack. Bioglassess and arginine in toothpastes have the ability to form a barrier over exposed dentine, protecting it from mechanical and chemical attack. The objective of this study was to evaluate the protection offered by three toothpastes containing Calcium-Sodium-Phosphosilicate (Novamin®), Arginine (Pro-Argin®) and Fluoro-Calcium-Phospho-Silicate (BioMin®) using nano-mechanical characterisation in a hydrated state.
Methods: 30 Bovine dentine discs were equally divided into 3 toothpaste treatment groups. Discs were brushed twice daily for two minutes over 7 days and kept in artificial saliva (AS) between brushing. 20 indents were performed on each disc prior to brushing to form a baseline and after 7 days of brushing. Load control indentation was performed using a 5μm radius spherical diamond tip with maximum penetration depth of 700nm to avoid substrate/dentine influences, at a rate of 0.5mN/s to a maximum load of 10mN which was held for 60s to allow for creep run out. A unique liquid cell set up was used to allow testing in presence of AS. After 7 days of brushing the discs were cross sectioned to measure the thickness of the layers using SEM. The thickness for Pro-Argin®, Novamin® and BioMin® were 9µm, 13µm and 8µm respectively.
Results: After brushing, the layers formed significantly increased the hardness and modulus for all treatment groups (p<0.05). Pro-Argin® formed a significantly harder and stiffer layer compared to Novamin® and BioMin® (p<0.05). There was no significant difference between Novamin® and BioMin® (p >0.05).
Conclusions: All 3 toothpastes formed layers which were harder than dentine.The most improvement was seen by Pro-Argin® which formed the hardest layer compared to the other two treatment groups which may offer the best protection against damage. Results showed no significant difference between Novamin® and BioMin®.
Methods: 30 Bovine dentine discs were equally divided into 3 toothpaste treatment groups. Discs were brushed twice daily for two minutes over 7 days and kept in artificial saliva (AS) between brushing. 20 indents were performed on each disc prior to brushing to form a baseline and after 7 days of brushing. Load control indentation was performed using a 5μm radius spherical diamond tip with maximum penetration depth of 700nm to avoid substrate/dentine influences, at a rate of 0.5mN/s to a maximum load of 10mN which was held for 60s to allow for creep run out. A unique liquid cell set up was used to allow testing in presence of AS. After 7 days of brushing the discs were cross sectioned to measure the thickness of the layers using SEM. The thickness for Pro-Argin®, Novamin® and BioMin® were 9µm, 13µm and 8µm respectively.
Results: After brushing, the layers formed significantly increased the hardness and modulus for all treatment groups (p<0.05). Pro-Argin® formed a significantly harder and stiffer layer compared to Novamin® and BioMin® (p<0.05). There was no significant difference between Novamin® and BioMin® (p >0.05).
Conclusions: All 3 toothpastes formed layers which were harder than dentine.The most improvement was seen by Pro-Argin® which formed the hardest layer compared to the other two treatment groups which may offer the best protection against damage. Results showed no significant difference between Novamin® and BioMin®.