IADR Abstract Archives
Antimicrobial Properties of Dental Acrylics Containing Ag-FAU Zeolite
Objectives: The aim of the present work is to fabricate dentures containing antimicrobial silver (Ag) zeolites, which can be re-charged with Ag upon silver depletion. The objectives are two-fold; firstly, to test the antimicrobial activity of dental acrylic polymers containing silver zeolites against oral microorganisms, namely Streptococcus mutans, Fusobacterium nucleatum and Candida albicans (x2), and secondly, to test the Ag release in distilled water over 2 months.
Methods: In total, four dental acrylic resins were prepared, dental acrylic (blank), dental acrylic containing Na-FAU, AgNO3 treated acrylic and AgNO3 treated dental acrylic containing Na-FAU (to charge it with silver). Dental acrylic surfaces were incubated at 37 °C under aerobic conditions for Candida albicans and under anaerobic conditions for S. mutans and F. nucleatum (approx. 106 CFU/m). Immediately after inoculation, the coupons were placed individually into 10 mL neutralizing agent to halt the antimicrobial effect of silver and vortexed for 30 s and the CFU/ml were established. All coupons were incubated for 0, 1 and 5 h at 37 °C prior to testing.
Results: It was determined that dental acrylic resin containing silver-zeolite showed antimicrobial efficacy against C. albicans and S. mutans. Antimicrobial efficacy of the acrylic resin coupons was still evident after 45 days of storage. After 60 days, the antimicrobial efficacy was lost indicating that Ag needs recharging after 45 days to restore the resin’s initial activity. Dental acrylics treated with AgNO3 showed no activity after an initial period of 5 days, during which adsorbed silver was removed from the surfaces. All silver-charged acrylics containing zeolites were tested after removal of this adsorbed silver, i.e. after 5 days of incubation in distilled water.
Conclusions: Dental acrylic containing antimicrobial Ag zeolites showed antimicrobial properties over a period of 45 days. Silver can be recharged into the zeolite-loaded resin upon silver depletion.
Methods: In total, four dental acrylic resins were prepared, dental acrylic (blank), dental acrylic containing Na-FAU, AgNO3 treated acrylic and AgNO3 treated dental acrylic containing Na-FAU (to charge it with silver). Dental acrylic surfaces were incubated at 37 °C under aerobic conditions for Candida albicans and under anaerobic conditions for S. mutans and F. nucleatum (approx. 106 CFU/m). Immediately after inoculation, the coupons were placed individually into 10 mL neutralizing agent to halt the antimicrobial effect of silver and vortexed for 30 s and the CFU/ml were established. All coupons were incubated for 0, 1 and 5 h at 37 °C prior to testing.
Results: It was determined that dental acrylic resin containing silver-zeolite showed antimicrobial efficacy against C. albicans and S. mutans. Antimicrobial efficacy of the acrylic resin coupons was still evident after 45 days of storage. After 60 days, the antimicrobial efficacy was lost indicating that Ag needs recharging after 45 days to restore the resin’s initial activity. Dental acrylics treated with AgNO3 showed no activity after an initial period of 5 days, during which adsorbed silver was removed from the surfaces. All silver-charged acrylics containing zeolites were tested after removal of this adsorbed silver, i.e. after 5 days of incubation in distilled water.
Conclusions: Dental acrylic containing antimicrobial Ag zeolites showed antimicrobial properties over a period of 45 days. Silver can be recharged into the zeolite-loaded resin upon silver depletion.