IADR Abstract Archives
Quantification of Mineral Deposits on Root Caries Using Dental Varnish
Objectives: The objective of this in vitro study was to quantify the amount of mineral change in demineralised dentin after application of three different dental varnishes containing fluoride either with CPP-ACP or Bioglass or fluoride only.
Methods: A total of 12 extracted human sound mandibular premolar specimens were coated with an acid-resistant varnish leaving a 2 × 3 mm window at the outer root surface. These root specimens were randomly divided into four groups, and separately subjected to a demineralising cycle with pH 4.8 for 5 days to create artificial caries-like lesions. Each sample was imaged using quantitative X-ray Microtomography (XMT) at a 15 µm voxel size. Each group then received one of the following treatments: Test A, the demineralised area painted by dental varnish containing fluoride and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP, MI varnish, GC Europe). Test B, fluoride and bioglass (NUPRO Experimental, DENTSPLY). The positive control, fluoride only (NUPRO, DENTSPLY), and the negative control received no treatment. These samples were kept in a deionised water for 12 hours. The thin layer of varnish was then removed. All specimens including the non-varnish group were subjected to a second demineralising cycle with pH 5.5 for 5 days. The final XMT imaging was carried out following the second demineralising cycle. The deposited mineral contents were analysed through the demineralised area to quantify the change in mineral following varnish application.
Results: The quantitative XMT assessment confirmed a significant increase in mineral content following the varnish application (p≤0.05) in all treatment cases. A significant decrease in mineral content was observed in the non-varnish group (p≤0.05).
Conclusions: There is promising evidence of a remineralisation effect with dental fluoride varnish on dentin in all cases, and the ability of this varnish to resist the demineralisation during acidic conditions in a laboratory setting.
Methods: A total of 12 extracted human sound mandibular premolar specimens were coated with an acid-resistant varnish leaving a 2 × 3 mm window at the outer root surface. These root specimens were randomly divided into four groups, and separately subjected to a demineralising cycle with pH 4.8 for 5 days to create artificial caries-like lesions. Each sample was imaged using quantitative X-ray Microtomography (XMT) at a 15 µm voxel size. Each group then received one of the following treatments: Test A, the demineralised area painted by dental varnish containing fluoride and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP, MI varnish, GC Europe). Test B, fluoride and bioglass (NUPRO Experimental, DENTSPLY). The positive control, fluoride only (NUPRO, DENTSPLY), and the negative control received no treatment. These samples were kept in a deionised water for 12 hours. The thin layer of varnish was then removed. All specimens including the non-varnish group were subjected to a second demineralising cycle with pH 5.5 for 5 days. The final XMT imaging was carried out following the second demineralising cycle. The deposited mineral contents were analysed through the demineralised area to quantify the change in mineral following varnish application.
Results: The quantitative XMT assessment confirmed a significant increase in mineral content following the varnish application (p≤0.05) in all treatment cases. A significant decrease in mineral content was observed in the non-varnish group (p≤0.05).
Conclusions: There is promising evidence of a remineralisation effect with dental fluoride varnish on dentin in all cases, and the ability of this varnish to resist the demineralisation during acidic conditions in a laboratory setting.