Objective: To compare the amount of plaque removed by two manual toothbrushes following a single brushing, with particular emphasis on hard to clean areas such as the back teeth. Method: This two-group randomised parallel design study consisted of 2 visits over two weeks with 85 subjects. At visit 1, subjects were randomly allocated either Brush 1 (a brush designed to allow easy access to the back teeth) or Brush 2 (a positive control marketed toothbrush) to use at home for at least 4 days before visit 2. Subjects refrained from brushing for 24 hours prior to the second visit, when their plaque was assessed before (PRE) and after brushing (POST) with their allocated brush using modified Navy index (MNI). Results: The mean PRE plaque scores recorded for each brush were not significantly different. The mean POST plaque scores for whole-mouth, interproximal sites and the back teeth were statistically significantly lower for Brush 1 than Brush 2, (where p<0.05). The increase in plaque removal obtained from using Brush 1 versus brush 2 was 15% for the whole mouth, 16% for the interproximal sites and 17% for the back teeth. There was no significant difference in brushing times between the two brushes. Conclusions: Brush 1 showed superior plaque removal efficacy throughout the whole mouth and in hard to clean areas such as the back teeth.