IADR Abstract Archives

Dentine infiltration with nanoparticles to promote remineralisation

Objectives: To specify the ability of silica and hydroxyapatite (HA) nanoparticles to infiltrate demineralised dentine. Also, to investigate the contribution of the nano-particulate infiltration in encouraging remineralisation of demineralised dentine.

Methods: Dentine blocks were obtained from intact human premolars. One sound dentine block was assigned as a positive control. The remaining dentine blocks were demineralised with 4N formic acid. Then they were divided into 6 groups (groups A-F). Each group consisted of 4 specimens. The first specimen of each group was immersed in a commercial nano-HA solution (HAcom) (PlasmaBiotal LTD, UK), the second in an experimental nano-HA solution (HAexp), the third in a colloidal silica solution (SiO2), while the fourth was kept as a non-infiltrated control. Transmission electron microscopy (TEM) was used to visually determine the presence of nanoparticles within the demineralised collagen matrix of group A specimens. Group B specimens were examined with energy dispersive x-ray (EDX) to evaluate the degree of infiltration by quantifying the concentrations of calcium (Ca), phosphorus (P) and silicon (Si). Groups C to F were immersed in a remineralising solution for 1, 4, 8 and 12 weeks. The levels of Ca, P and Si were measured after each respective time period.

Results: TEM images confirmed the presence of nanoparticles in the demineralised dentine matrix in the cases of (HAexp) and (SiO2) solutions. EDX confirmed that all nano-particulate solutions tested had infiltrated human dentine. (HAexp) solution increased the P and Ca levels up to 65.1% and 55.31% of the sound dentine control respectively. After immersion in the remineralising solution, specimens previously infiltrated with (SiO2) had 15 times higher concentration in P and 6 times higher in Ca, when compared to non-infiltrated specimens.

Conclusion: Silica and hydroxyapatite nano-particulate solutions are capable of infiltrating demineralised human dentine. Given the right environment, nanoparticles can promote dentine remineralisation.

British Division Meeting
2009 British Division Meeting (Glasglow, Scotland)
Glasglow, Scotland
Scientific Groups
  • Besinis, Alexandros  ( University of Sheffield, Sheffield, N/A, United Kingdom )
  • Noort, R.v.  ( University of Sheffield, Sheffield, N/A, United Kingdom )
  • Martin, N.  ( University of Sheffield, Sheffield, N/A, United Kingdom )
  • Oral Session