Objectives: The aim of this study was to investigate the effect of P-15 modified ABM scaffolds on human dental pulp stem cell (hDPSCs) growth and differentiation along an osteogenic pathway.
Methods: hDPSCs were seeded on to ABM and ABM-P-15 scaffolds and cultured in basal media for up to three weeks. The cells on the scaffold were then analysed using live/dead fluorescent markers, routine histology and confocal and scanning electron microscopy. In addition, the effect of P-15 on BMP production by hDPSCs was analysed by C2C12 promyloblasts induction assay.
Results: Histological analysis revealed that hDPSCs cultured on ABM-P-15 scaffolds formed extensive cell bridges between the scaffold particles. Alkaline phosphatase expression and BMP-2 production were also increased when hDPSCs were cultured on ABM-P-15 scaffolds compared with the ABM only controls.
Conclusions: P-15 absorbed ABM scaffolds appear to provide an ideal biomimetic microenvironment for human dental pulp stem cell growth and differentiation along the osteogenic lineage, offering new candidate material for bone regeneration.
This project was supported by CeraPedics LLC.