Methods: Extracted bovine dentine was processed mechanically and chemically with inorganic and organic solvents and sterilised. The in vitro biocompatibility on human gingival fibroblasts was assessed by the Alamar Blue assay and the in vivo biocompatibility was evaluated by the implantation of the processed dentine into the rat femur.
Results: Dentine product showed excellent biocompatibility in vitro and stimulated new bone formation and completely incorporated into the new bone in vivo.
Conclusions: Processed bovine dentine has the potential to be used as a suitable bone substitute in bone repair and regeneration.