IADR Abstract Archives
Biocompatibility of Surface-Modified Titanium Implants with Silver and Hydroxyapatite Nanoparticles
Objectives: To examine the stability of silver and hydroxyapatite nanocoatings applied to the surface of titanium dental implants in cell culture media. Also, to investigate the biocompatibility of these surface-modified dental implants.
Methods: Ti6Al4V discs were coated with silver nanoparticles (Ag NPs), silver and hydroxyapatite nanoparticles (Ag+nHA) or microparticles (Ag+mHA). The silver and HA coatings were applied using the electroplating and sintering techniques respectively. The stability of the silver-hydroxyapatite nanocoatings was tested in different cell culture media (physiological saline, DMEM, human osteoblast growth medium; HOB) using inductively coupled plasma mass spectrometry (ICP-MS). The biocompatibility of the coatings was tested with primary human osteoblasts in 24-well microplates (n = 9 discs/treatment; 20,000 cells/well). The cell viability was assessed by measuring the LDH and ALP activities as well as the cell protein content over 72h. The cell morphology was investigated using scanning electron microscopy (SEM). Ag and cell electrolyte (Na+, K+) concentrations in the media were measured using (ICP-MS).
Results: SEM and energy dispersive X-ray spectroscopy (EDS) confirmed that Ti6Al4V discs were successfully coated. Electron microscopy confirmed that the primary particle size of Ag, nHA and mHA was 111.58±14.99 nm, 23.90±1.49 nm and 4.72±0.38 µm respectively. Metal analysis showed that silver coatings remain stable in all media tested (<0.57%), but dissolution of Ag NPs in DMEM was 4-fold higher compared to HOB media. The presence of FBS in the media was found to increase the silver release from the nanocoatings significantly. The findings of the LDH, ALP and protein assays agreed that viability of the human osteoblasts cells adherent to the implants was lower for the Ag+mHA coated specimens compared to Ag+nHA.
Conclusions: Ag and HA NPs formed stable coatings on titanium implants. Implants coated with Ag+HA NPs maintained a higher degree of biocompatibility compared to those coated with Ag+mHA suggesting a benefit for clinical use.
Methods: Ti6Al4V discs were coated with silver nanoparticles (Ag NPs), silver and hydroxyapatite nanoparticles (Ag+nHA) or microparticles (Ag+mHA). The silver and HA coatings were applied using the electroplating and sintering techniques respectively. The stability of the silver-hydroxyapatite nanocoatings was tested in different cell culture media (physiological saline, DMEM, human osteoblast growth medium; HOB) using inductively coupled plasma mass spectrometry (ICP-MS). The biocompatibility of the coatings was tested with primary human osteoblasts in 24-well microplates (n = 9 discs/treatment; 20,000 cells/well). The cell viability was assessed by measuring the LDH and ALP activities as well as the cell protein content over 72h. The cell morphology was investigated using scanning electron microscopy (SEM). Ag and cell electrolyte (Na+, K+) concentrations in the media were measured using (ICP-MS).
Results: SEM and energy dispersive X-ray spectroscopy (EDS) confirmed that Ti6Al4V discs were successfully coated. Electron microscopy confirmed that the primary particle size of Ag, nHA and mHA was 111.58±14.99 nm, 23.90±1.49 nm and 4.72±0.38 µm respectively. Metal analysis showed that silver coatings remain stable in all media tested (<0.57%), but dissolution of Ag NPs in DMEM was 4-fold higher compared to HOB media. The presence of FBS in the media was found to increase the silver release from the nanocoatings significantly. The findings of the LDH, ALP and protein assays agreed that viability of the human osteoblasts cells adherent to the implants was lower for the Ag+mHA coated specimens compared to Ag+nHA.
Conclusions: Ag and HA NPs formed stable coatings on titanium implants. Implants coated with Ag+HA NPs maintained a higher degree of biocompatibility compared to those coated with Ag+mHA suggesting a benefit for clinical use.