IADR Abstract Archives
In vitro effects of a novel hydroxyapatite-fluoride agent (IWI2) upon enamel repair and remineralisation
Objectives: 1. To trial air abrasion to generate enamel surface defects of controlled extent matched to a control smooth enamel surface
2. To evaluate the repair and remineralisation of enamel surface defects and artificial white spot lesions (WSLs) using two variations of a novel remineralisation agent, IWI2 (Sylphar Ltd), against positive and negative controls
Methods: Polished human enamel specimens were allocated to six treatment groups: two IWI2 variations, casein phosphopeptide amorphous calcium phosphate (CPP-ACP) (GC Corp.), 1500ppm fluoride gel, a placebo gel and a negative control. Alumina powder air abrasion was used to generate enamel surface defects. Repair was evaluated using SEM, confocal profilometry, and Raman spectroscopy.
Optical coherence tomography was used to evaluate the remineralisation of a separate artificial white spot lesion model.
Specimens were maintained in artificial saliva at 37.5o C and material applied to samples for 20 minutes for 5 consecutive days. They were assessed at 0, 1, 2, 5 and 30 days.
Data were analysed using one-way analysis of variance tests (p = 0.05).
Results: SEM and confocal profilometry showed significantly more infill of enamel surface defects with application of IWI2 than the negative controls. Similar results were achieved with CPP-ACP.
One of the IWI2 formulations, IW37, resulted in significantly more mineralisation of enamel surface defects. Fluoride performed similarly well, but no other treatment group significantly differed from the negative control.
Furthermore, IW37 and CPP-ACP resulted in a significant reduction in OCT subsurface light scattering of WSLs relative to other treatment groups. No other treatment group was better than the negative control.
Conclusions: Air abrasion is a suitable method for creating enamel surface defects. Both IWI2 formulations enhanced enamel surface repair; and IW37 contributed to the mineralisation of both models.
2. To evaluate the repair and remineralisation of enamel surface defects and artificial white spot lesions (WSLs) using two variations of a novel remineralisation agent, IWI2 (Sylphar Ltd), against positive and negative controls
Methods: Polished human enamel specimens were allocated to six treatment groups: two IWI2 variations, casein phosphopeptide amorphous calcium phosphate (CPP-ACP) (GC Corp.), 1500ppm fluoride gel, a placebo gel and a negative control. Alumina powder air abrasion was used to generate enamel surface defects. Repair was evaluated using SEM, confocal profilometry, and Raman spectroscopy.
Optical coherence tomography was used to evaluate the remineralisation of a separate artificial white spot lesion model.
Specimens were maintained in artificial saliva at 37.5o C and material applied to samples for 20 minutes for 5 consecutive days. They were assessed at 0, 1, 2, 5 and 30 days.
Data were analysed using one-way analysis of variance tests (p = 0.05).
Results: SEM and confocal profilometry showed significantly more infill of enamel surface defects with application of IWI2 than the negative controls. Similar results were achieved with CPP-ACP.
One of the IWI2 formulations, IW37, resulted in significantly more mineralisation of enamel surface defects. Fluoride performed similarly well, but no other treatment group significantly differed from the negative control.
Furthermore, IW37 and CPP-ACP resulted in a significant reduction in OCT subsurface light scattering of WSLs relative to other treatment groups. No other treatment group was better than the negative control.
Conclusions: Air abrasion is a suitable method for creating enamel surface defects. Both IWI2 formulations enhanced enamel surface repair; and IW37 contributed to the mineralisation of both models.
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