IADR Abstract Archives

The effect of toothbrush abrasion force on dentine hypersensitivity in-vitro

Objectives: This study investigated the effect of tooth brushing force on changes in dentine tubule patency in an erosion-toothbrush abrasion model.
Methods: 60 dentine samples were prepared and polished with an artificial smear layer and divided randomly into control (no tooth brushing), 100g, 200g or 400g brushing force groups. Tandem Scanning Microscopy (TSM) (x40) imaged the centre of each sample. Samples were immersed in 3:1 artificial saliva/NaF 1450ppm and either brushed, using a standard protocol (p35 soft tooth brush; 120 strokes) at the specified force, or not brushed. Then samples were subjected to an agitated acid challenge (0.3% citric acid pH2.6 for 2 minutes). Finally, samples were re-brushed using the same protocol. TSM images (x40) were retaken after each stage. Previously validated software calculated the numbers of patent dentine tubules. Profilometry was used to determine tooth wear.
Results: At baseline, mean patent tubules in all samples were 188 per image (SD 54) with no significant inter-group differences. Following the first brushing cycle, mean patent tubules from baseline decreased using 100g to 150 (SD 32) (p<0.01) and increased using 400g to 215 (SD 45) (p=0.02). Differences in patent tubules between 100g and 400g groups were significant after first brushing (p<0.001). Following acid challenge, mean patent tubules increased to 218 (SD 40) in all samples (p<0.01) with no significant inter-group differences. Following further brushing, mean patent tubules decreased using 100g to 175 (SD 72) (p<0.01), but increased with 400g to 232 (SD 52). Differences in patent tubules between 100g and 400g groups were significant after second brushing (p<0.001). Tooth wear on all samples was a mean 2µm (SD 2µm).
Conclusions: Tooth wear was minimal (2µm+2µm) and likely to be within the smear layer (10µm+5µm). At higher brushing forces (400g), more tubules were exposed whereas at lower brushing forces (100g), tubule patency decreases even post-acidic challenge.
British Division Meeting
2015 British Division Meeting (Cardiff, United Kingdom)
Cardiff, United Kingdom
2015
190
Prosthodontics Research
  • Sehmi, Harminder  ( King's College London , London , United Kingdom )
  • Bartlett, David  ( King's College London , London , United Kingdom )
  • Olley, Ryan  ( King's College London , London , United Kingdom )
  • NONE
    Oral Session
    Evaluation of interventions
    Wednesday, 09/16/2015 , 11:30AM - 01:00PM