Odontogenic differentiation of human dental pulp stem cells (hDPSCs) under strontium treatment
Objectives: As a trace element similar to calcium, strontium(Sr) is commonly used in dental applications. For example it has been doped in toothpaste to treat dentine hypersensitivity, or in glass ionomer cement in dental restorative materials. However, the biological effects of Sr on the dentine-pulp complex require further elucidation. The use of human dental pulp stem cells (hDPSCs) in tooth regeneration is favoured as they have the ability to generate the components of dentine-pulp complex. The aims of this study are to investigate the influences of strontium on the behaviour of human dental pulp stem cells (hDPSCs). Methods: hDPSCs were treated with or without Sr, then cell proliferation and alkaline phosphatise activity (ALP) was quantified by DNA flourometric assay and ALP activity assay respectively. Odontogenic differentiation related gene expression: DSPP, DMP-1, ALP and type I collagen (COL-1) was assessed by quantitative polymerase chain reaction (qPCR). Western-blot and immunocytochemical staining were used to determine the level and location of DSPP and DMP-1. Alizarin Red S staining was used to quantify the degree of Sr mediated mineralization. Results: Low doses of Sr (≤ 2.5mM) significantly influence proliferation ability and ALP activity of DPSCs, while 5mM Sr treatment induces cell proliferation only. The Sr exposure groups displayed significant increase of DSPP and DMP-1 expression compared with negative control group at mRNA and protein level. Sr also stimulated mineralization. DMP-1 appeared to be ubiquitous in its staining pattern both in cytosol and nucleus, while DSPP is observed to be localised in perinuclear structure. Conclusions: This study provides the first evidence that Sr at specific doses significantly influences proliferation, odontogenic differentiation and mineralization of hDPSCs. Therefore Sr is a promising candidate for dental hard tissue regeneration. It is hoped this information could pave the way for using of strontium in dental applications for dental tissue regeneration.
Division: British Division Meeting
Meeting:2015 British Division Meeting (Cardiff, United Kingdom) Location: Cardiff, United Kingdom
Year: 2015 Final Presentation ID:95 Abstract Category|Abstract Category(s):Pulp Biology & Regeneration Research
Authors
Huang, Mei
( Queen Mary University of London
, London
, United Kingdom
)
Hill, Robert
( Queen Mary University of London
, London
, United Kingdom
)
Rawlinson, Simon
( Queen Mary University of London
, London
, United Kingdom
)