IADR Abstract Archives
Biocompatibility and biomineralization assessment of bioceramic, epoxy-resin based and calcium hydroxide root canal sealers.
Objectives: To evaluate in vivo biological response and biomineralization capacity of the endodontic sealers Smartpaste Bio, Sealapex and Acroseal.
Methods: Subcutaneous implants in 40 Wistar rats were performed. Analysis was at 7, 15, 30 and 60 days experimental periods (10 animals for each time period). Each animal received four implants, three polyethylene tubes with the sealers in test and one empty tube as control. After each post-operative period animals were euthanized and the polyethylene tubes, along with surrounding tissue were removed and fixed. In order to histologically analysis fibrous capsule thickness, inflammatory infiltrate and mineralization, the pieces were included in historesin and stained in HE, Von Kossa or remained without staining for observation under polarized light. The results were statistically analyzed by Kruskal-Wallis and Dunn’s test (p<0,05).
Results: All sealers promoted moderate inflammatory reaction at initial periods. Smartpaste Bio presented the lowest inflammatory reaction at 15 days period (p<0.05). Sealapex induced higher mineralization, followed by Smartpaste Bio. Acroseal showed no mineralization areas.
Conclusions: At the end of the experiment, all tested sealers Smartpaste Bio, Sealapex and Acroseal presented biocompatibility. Smartpaste Bio and Sealapex induced biomineralization.
Methods: Subcutaneous implants in 40 Wistar rats were performed. Analysis was at 7, 15, 30 and 60 days experimental periods (10 animals for each time period). Each animal received four implants, three polyethylene tubes with the sealers in test and one empty tube as control. After each post-operative period animals were euthanized and the polyethylene tubes, along with surrounding tissue were removed and fixed. In order to histologically analysis fibrous capsule thickness, inflammatory infiltrate and mineralization, the pieces were included in historesin and stained in HE, Von Kossa or remained without staining for observation under polarized light. The results were statistically analyzed by Kruskal-Wallis and Dunn’s test (p<0,05).
Results: All sealers promoted moderate inflammatory reaction at initial periods. Smartpaste Bio presented the lowest inflammatory reaction at 15 days period (p<0.05). Sealapex induced higher mineralization, followed by Smartpaste Bio. Acroseal showed no mineralization areas.
Conclusions: At the end of the experiment, all tested sealers Smartpaste Bio, Sealapex and Acroseal presented biocompatibility. Smartpaste Bio and Sealapex induced biomineralization.