Citotoxicity of Dental Alloys Obtained From Two Casting Methods

Objectives: There are many dental alloys that are placed into contact with oral tissues. Then, the aim of this study was to compare citotoxicity of Ni-Cr-Be, Ni-Cr, Co-Cr-Mo, Co-Cr-Mo-W, Ni-Cr-Ti (Tilite e Vi-Star), Ti-6Al-4V alloys and CP Ti, using keratinocytes cell line SCC9 (ATCC). Methods: Disks (13x4mm) were cast in Ni-Cr-Be, Ni-Cr, Co-Cr-Mo, Co-Cr-Mo, Co-Cr-Mo-W, and Ni-Cr-Ti, by plasma (P) under argon atmosphere, injected by vacuum-pressure; and by oxygen gas flame (C), injected by centrifugation. The CP Ti and Ti-6Al-4V were cast by plasma (P), under argon atmosphere, injected by vacuum-pressure. After casting, the disks were polished, cleaned and autoclaved. SCC9 cell were cultured (0.5 X 10⁴ cell/well) in culture media DMEM/HF12 supplemented, on the disks; and empty wells were used as a control. After the period of 48 hours in culture, the cells were harvested and counted in hemacytometer. Results: The results, expressed as media and standard deviation of the cell number X 10⁴/well, were: Control: 0.40±0.20; Ni-Cr-Be(P): 0.30±0.27; Ni-Cr-Be(C): 0.27±0.25; Ni-Cr(P): 0.30±0.27; Ni-Cr(C): 0.27±0.25; Co-Cr-Mo(P): 0.20±0.18; Co-Cr-Mo(C): 0.23±0.25; Co-Cr-Mo-W(P): 0.20±0.17; Co-Cr-Mo-W(C): 0.30±0.22; Tilite(P): 0.27±0.09; Tilite(C): 0.40±0.19; Vi-Star(P): 0.33±0.12; Vi-Star(C): 0.13±0.14; CP Ti: 0.33±0.17; Ti-6Al-4V: 0.20±0.14. The comparison of data by ANOVA showed that there was no significant difference among the groups (p=0.686) and between both casting methods (p=0.839). Conclusion: The data suggest that the alloys and casting methods are not cytotoxic to oral epithelium cells. FAPESP 03/01775-4